Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,867,615	G→A	T429I (ACC→ATC)	ygcU ←	predicted FAD containing dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,867,615	0	G	A	100.0%	166.8 / NA	48	T429I (ACC→ATC)	ygcU	predicted FAD containing dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/42);  total (6/42)

CTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTC  >  minE/1867552‑1867667                                                                |                                                     cTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttctt                                                <  1:441650/70‑1 (MQ=255) cTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttctt                                                <  1:2799985/70‑1 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatg                                        <  1:833899/70‑1 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatg                                        <  1:1076309/70‑1 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatg                                        <  1:498268/70‑1 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatg                                        <  1:360137/70‑1 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatg                                        <  1:2412324/70‑1 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatg                                        <  1:2276348/70‑1 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatg                                        <  1:2006606/70‑1 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatg                                        <  1:2005122/70‑1 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatg                                        <  1:1566378/70‑1 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatg                                        <  1:1520769/70‑1 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatg                                        <  1:133355/70‑1 (MQ=255)         aaCGCGATGTTTACCGATACCGGGGTGGTGCACCATCGAACCGCCGAGCCGAATGATTTCTTCACAgatg                                        <  1:1103560/70‑1 (MQ=255)          aCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatg                                        <  1:2999236/69‑1 (MQ=255)                    tACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAg                             <  1:1239220/70‑1 (MQ=255)                    tACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAg                             <  1:2843248/70‑1 (MQ=255)                    tACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAg                             <  1:2324734/70‑1 (MQ=255)                    tACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAg                             <  1:2690831/70‑1 (MQ=255)                    tACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAg                             <  1:1301707/70‑1 (MQ=255)                        gATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGAt                        <  1:1597900/71‑1 (MQ=255)                        gATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGAt                        <  1:761243/71‑1 (MQ=255)                        gATACCGTGGTGGTGCACCATCGAACCGCCGACGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGAt                        <  1:2276729/71‑1 (MQ=255)                                tgtggtgCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatg                                        <  1:2843158/46‑1 (MQ=255)                                 gtggtgCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACt               >  1:1747829/1‑71 (MQ=255)                                 gtggtgCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACt               >  1:489245/1‑71 (MQ=255)                                  ttgtgCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatg                                        <  1:2392048/43‑1 (MQ=255)                                           aTCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc     <  1:433044/71‑1 (MQ=255)                                           aTCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc     <  1:1519447/71‑1 (MQ=255)                                           aTCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc     <  1:729795/71‑1 (MQ=255)                                           aTCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc     <  1:645005/71‑1 (MQ=255)                                           aTCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc     <  1:825927/71‑1 (MQ=255)                                           aTCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc     <  1:1271359/71‑1 (MQ=255)                                           aTCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc     <  1:480597/71‑1 (MQ=255)                                           aTCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc     <  1:1512334/71‑1 (MQ=255)                                           aTCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc     <  1:441112/71‑1 (MQ=255)                                           aTCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc     <  1:1261694/71‑1 (MQ=255)                                           aTCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc     <  1:3277953/71‑1 (MQ=255)                                           aTCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc     <  1:3095556/71‑1 (MQ=255)                                           aTCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc     <  1:3017105/71‑1 (MQ=255)                                           aTCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc     <  1:2203279/71‑1 (MQ=255)                                           aTCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc     <  1:2987824/71‑1 (MQ=255)                                           aTCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc     <  1:2936725/71‑1 (MQ=255)                                           aTCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc     <  1:2890905/71‑1 (MQ=255)                                              gAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCctc  >  1:2619687/1‑71 (MQ=255)                                              gAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCctc  >  1:810700/1‑71 (MQ=255)                                              gAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCctc  >  1:1118528/1‑71 (MQ=255)                                              gAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCctc  >  1:2901280/1‑71 (MQ=255)                                                                |                                                     CTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTC  >  minE/1867552‑1867667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑