Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,207,601	A→T	intergenic (‑291/+139)	tldD ← / ← yhdP	predicted peptidase/conserved membrane protein, predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,207,601	0	A	T	100.0%	47.7 / NA	20	intergenic (‑291/+139)	tldD/yhdP	predicted peptidase/conserved membrane protein, predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (0/20);  total (0/20)

TTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCC  >  minE/2207582‑2207652                    |                                                    ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:2462054/71‑1 (MQ=21) ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:834969/71‑1 (MQ=21) ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:790964/71‑1 (MQ=21) ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:596948/71‑1 (MQ=21) ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:3271502/71‑1 (MQ=21) ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:3211855/71‑1 (MQ=21) ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:3101805/71‑1 (MQ=21) ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:3008769/71‑1 (MQ=21) ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:2935773/71‑1 (MQ=21) ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:1005802/71‑1 (MQ=21) ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:2293680/71‑1 (MQ=21) ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:2261964/71‑1 (MQ=21) ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:1951447/71‑1 (MQ=21) ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:1924085/71‑1 (MQ=21) ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:173538/71‑1 (MQ=21) ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:1320879/71‑1 (MQ=21) ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:1087200/71‑1 (MQ=21) ttCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCCGCCGGATGCGGCGTGAACGcc  <  1:1891246/71‑1 (MQ=11)  tCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:3080763/70‑1 (MQ=25)               cTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc  <  1:2353612/57‑1 (MQ=255)                    |                                                    TTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCC  >  minE/2207582‑2207652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑