Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,355,388 C→G A271P (GCC→CCC)  yihE ← predicted kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,355,3880CG100.0% 386.4 / NA 104A271P (GCC→CCC) yihEpredicted kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (82/22);  total (82/22)

Only 100 of 104 total aligned reads displayed.

CGGGAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGCGCGTAAAGGTTCAATCAGTCCGATTTCAGCGGTGTCGAACTCGCTAAATTCTTCAT  >  minE/2355326‑2355444
                                                              |                                                        
cGGGAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTaaa                                                   <  1:227486/70‑1 (MQ=255)
cGGGAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTaaa                                                   <  1:3195483/70‑1 (MQ=255)
cGGGAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTaaa                                                   <  1:951118/70‑1 (MQ=255)
cGGGAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTaaa                                                   <  1:2146911/70‑1 (MQ=255)
cGGGAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTaaa                                                   <  1:2221323/70‑1 (MQ=255)
cGGGAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTaaa                                                   <  1:6482/70‑1 (MQ=255)
cGGGAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTaaa                                                   <  1:1747880/70‑1 (MQ=255)
cGGGAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTaaa                                                   <  1:680825/70‑1 (MQ=255)
cGGGAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTaaa                                                   <  1:1707664/70‑1 (MQ=255)
cGGGAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTaaa                                                   <  1:3169326/70‑1 (MQ=255)
 gggAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAgg                                                 <  1:1727108/71‑1 (MQ=255)
 gggAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAgg                                                 <  1:1413942/71‑1 (MQ=255)
 gggAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAgg                                                 <  1:2437070/71‑1 (MQ=255)
 gggAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAgg                                                 <  1:814899/71‑1 (MQ=255)
 gggAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAgg                                                 <  1:1170189/71‑1 (MQ=255)
 gggAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAgg                                                 <  1:2787391/71‑1 (MQ=255)
 gggAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAgg                                                 <  1:3173426/71‑1 (MQ=255)
  ggAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAgg                                                 <  1:2035736/70‑1 (MQ=255)
  ggAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAgg                                                 <  1:240406/70‑1 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:667663/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:2257139/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:2306170/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:3048261/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:2444240/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:3197394/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:386374/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:35991/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:2961184/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:757546/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:2019805/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:1512028/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:127453/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:1290824/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:1345585/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:1371436/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:815363/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:939144/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:1640751/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:1067447/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:799397/1‑71 (MQ=255)
            aTCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:1878418/1‑71 (MQ=255)
            aTCAGACCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTc                                      >  1:1062931/1‑71 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:2725884/1‑71 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:2696748/1‑71 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:1918302/1‑71 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:2589414/1‑71 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:2565134/1‑71 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:2528431/1‑71 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:2811627/1‑71 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:1181736/1‑71 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:871338/1‑71 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:2372357/1‑71 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:828261/1‑71 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:3068145/1‑71 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:1594091/1‑71 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:161187/1‑71 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:1615145/1‑71 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:735314/1‑71 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAtt                                 >  1:3102724/1‑70 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAtt                                 >  1:1055849/1‑70 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAtt                                 >  1:2917133/1‑70 (MQ=255)
                  ccAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGACCGAtt                                 >  1:2469566/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:3225397/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:354131/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:468359/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:590641/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:70540/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:77906/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:832074/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:974118/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:1062938/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:1306378/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:1355019/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:1279673/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:1779711/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:1796704/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:2109449/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:1112037/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:2278616/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:2334647/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:2367864/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:280482/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:2858389/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:2916070/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAttt                                >  1:3156484/1‑70 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAtt                                 >  1:514340/1‑69 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAtt                                 >  1:2504356/1‑69 (MQ=255)
                   cAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGAtt                                 >  1:3043368/1‑69 (MQ=255)
                                           taataaACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGATTTCAGCGGTGTCGAACTCGCTAAAttc       <  1:2216688/71‑1 (MQ=255)
                                           taataaACCAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGATTTCAGCGGTGTCGAACTCGCTAAAttc       <  1:2066499/71‑1 (MQ=255)
                                                  ccgaaCGCATGGGGCGTAAAGGTTCAATCAGTCCGATTTCAGCGGTGTCGAACTCGCTAAATTCTTCAt  >  1:575811/4‑69 (MQ=255)
                                                  ccAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGATTTCAGCGGTGTCGAACTCGCTAAATTCTTCAt  >  1:1043407/1‑69 (MQ=255)
                                                  ccAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGATTTCAGCGGTGTCGAACTCGCTAAATTCTTCAt  >  1:3170014/1‑69 (MQ=255)
                                                  ccAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGATTTCAGCGGTGTCGAACTCGCTAAATTCTTCAt  >  1:900792/1‑69 (MQ=255)
                                                  ccAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGATTTCAGCGGTGTCGAACTCGCTAAATTCTTCAt  >  1:1406637/1‑69 (MQ=255)
                                                  ccAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGATTTCAGCGGTGTCGAACTCGCTAAATTCTTCAt  >  1:1774551/1‑69 (MQ=255)
                                                  ccAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGATTTCAGCGGTGTCGAACTCGCTAAATTCTTCAt  >  1:2166441/1‑69 (MQ=255)
                                                  ccAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGATTTCAGCGGTGTCGAACTCGCTAAATTCTTCAt  >  1:2315853/1‑69 (MQ=255)
                                                  ccAAACGCATGGGGCGTAAAGGTTCAATCAGTCCGATTTCAGCGGTGTCGAACTCGCTAAATTCTTCAt  >  1:3253824/1‑69 (MQ=255)
                                                           tGGGGCGTAAAGGTTCAATCAGTCCGATTTCAGCGGTGTCGAACTCGCTAAAttc       <  1:842429/55‑1 (MQ=255)
                                                              |                                                        
CGGGAACGCGGGATCAGCCCAACGCCGCATTAGCCAGGCAAGATAATAAACCAAACGCATGGCGCGTAAAGGTTCAATCAGTCCGATTTCAGCGGTGTCGAACTCGCTAAATTCTTCAT  >  minE/2355326‑2355444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑