Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,364,103	A→G	W456R (TGG→CGG)	trkH ←	potassium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,364,103	0	A	G	100.0%	324.0 / NA	87	W456R (TGG→CGG)	trkH	potassium transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (69/18);  total (69/18)

GTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCC  >  minE/2364039‑2364160                                                                 |                                                          gTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:1906156/71‑1 (MQ=255) gTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:3061092/71‑1 (MQ=255) gTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:1254849/71‑1 (MQ=255) gTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:304941/71‑1 (MQ=255) gTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:2951131/71‑1 (MQ=255) gTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:1753627/71‑1 (MQ=255) gTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:1696773/71‑1 (MQ=255) gTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:247582/71‑1 (MQ=255) gTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:2356837/71‑1 (MQ=255) gTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:1936443/71‑1 (MQ=255) gTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:1536256/71‑1 (MQ=255) gTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:2236665/71‑1 (MQ=255) gTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:646961/71‑1 (MQ=255) gTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:1966503/71‑1 (MQ=255) gTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:767801/71‑1 (MQ=255)  tAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:1061810/70‑1 (MQ=255)   aaaGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:2118217/69‑1 (MQ=255)              gCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                     <  1:1355662/58‑1 (MQ=255)                      aaGCCCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:2259524/1‑69 (MQ=255)                      aaGACCTCGAGACGCCCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:252499/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTg                                     >  1:2303080/1‑66 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGt                                   >  1:400647/1‑68 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:1802488/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:2503379/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:2416431/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:2387882/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:1516582/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:2352942/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:2346589/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:2318037/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:2307170/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:307432/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:1521410/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:2193155/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:1653882/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:1919978/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:1981082/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:570917/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:2006445/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                  >  1:166336/1‑69 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACAGGGTTCATACTGGTa                                  >  1:2760195/1‑69 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCg                                                >  1:3041596/1‑51 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACt                                      >  1:1263879/1‑61 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGtt                             >  1:2109752/1‑70 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGtt                             >  1:608810/1‑70 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:2794952/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:872178/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:2962057/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:3102178/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:301991/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:3036464/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:2779832/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:527060/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:1236641/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:3938/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:388647/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:372105/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:3086387/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:3095518/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:2379190/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:1688368/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:866442/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:195627/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:1669506/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:202144/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:1625207/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:1607273/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:2250380/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:1771425/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:136045/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:2494009/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:1271348/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:616231/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:2554039/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:2557793/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:2635047/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                            >  1:2762944/1‑71 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTAGTAAAGTTa                            >  1:2732652/1‑71 (MQ=255)                                                      aaTCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCacc                    >  1:930572/1‑49 (MQ=255)                                                      aaTCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCTAAGCCCTGGccc  >  1:1986135/1‑69 (MQ=255)                                                      aaTCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGccc  >  1:3244339/1‑69 (MQ=255)                                                      aaTCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGccc  >  1:3063372/1‑69 (MQ=255)                                                      aaTCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGccc  >  1:2529135/1‑69 (MQ=255)                                                      aaTCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGccc  >  1:2191415/1‑69 (MQ=255)                                                      aaTCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGccc  >  1:1942234/1‑69 (MQ=255)                                                      aaTCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGccc  >  1:1265845/1‑69 (MQ=255)                                                      aaTCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGccc  >  1:1161086/1‑69 (MQ=255)                                                                 |                                                          GTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCC  >  minE/2364039‑2364160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑