Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,371,447	A→G	F214L (TTT→CTT)	fre ←	flavin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,371,447	0	A	G	100.0%	140.6 / NA	38	F214L (TTT→CTT)	fre	flavin reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/36);  total (2/36)

ATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGCCAGCG  >  minE/2371393‑2371512                                                       |                                                                  aTGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAAtt                                                   <  1:320036/71‑1 (MQ=255)              gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCaa                                      <  1:461594/71‑1 (MQ=255)              gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCaa                                      <  1:454347/71‑1 (MQ=255)              gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCaa                                      <  1:1829200/71‑1 (MQ=255)              gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCaa                                      <  1:373905/71‑1 (MQ=255)              gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCaa                                      <  1:1905149/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTGAAAAc                                   <  1:803491/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCaaagc                                   <  1:2446023/71‑3 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:2961498/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:1143046/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:310535/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:3190242/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:3284314/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:3293600/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:489492/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:529781/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:529784/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:642933/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:847941/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:966406/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:1788207/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:114217/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:1413639/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:1509239/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:1573201/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:1637918/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:1703149/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:1782990/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:2846987/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:1866546/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:2025950/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:2072704/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:2504330/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:2599563/71‑1 (MQ=255)                 aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:2636267/71‑1 (MQ=255)                  aaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:392383/70‑1 (MQ=255)                                                   cAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGCCAGCg  >  1:1329825/1‑70 (MQ=255)                                                   cAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGCCAGCg  >  1:2692128/1‑70 (MQ=255)                                                       |                                                                  ATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGCCAGCG  >  minE/2371393‑2371512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑