Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,450,298 A→G W78R (TGG→CGG)  yifE ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,450,2980AG100.0% 817.9 / NA 214W78R (TGG→CGG) yifEconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (77/137);  total (77/137)

Only 100 of 214 total aligned reads displayed.

ACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTC  >  minE/2450239‑2450364
                                                           |                                                                  
aCCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCt                                                          <  1:3201083/70‑1 (MQ=255)
aCCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCt                                                          <  1:1466919/70‑1 (MQ=255)
aCCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCt                                                          <  1:3000514/70‑1 (MQ=255)
aCCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCt                                                          <  1:824538/70‑1 (MQ=255)
aCCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCt                                                          <  1:2658023/70‑1 (MQ=255)
aCCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCt                                                          <  1:1930166/70‑1 (MQ=255)
aCCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCt                                                          <  1:1749524/70‑1 (MQ=255)
aCCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCt                                                          <  1:1182907/70‑1 (MQ=255)
aCCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCt                                                          <  1:351337/70‑1 (MQ=255)
aCCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCt                                                          <  1:682828/70‑1 (MQ=255)
 ccgccgGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCt                                                          <  1:1820265/69‑1 (MQ=255)
 ccgccgGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCt                                                          <  1:1364719/69‑1 (MQ=255)
                      cGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc                                   <  1:286288/71‑1 (MQ=255)
                      cGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc                                   <  1:30197/71‑1 (MQ=255)
                      cGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc                                   <  1:2724743/71‑1 (MQ=255)
                      cGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc                                   <  1:262053/71‑1 (MQ=255)
                      cGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc                                   <  1:2394707/71‑1 (MQ=255)
                      cGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc                                   <  1:7148/71‑1 (MQ=255)
                      cGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc                                   <  1:879324/71‑1 (MQ=255)
                      cGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc                                   <  1:2375001/71‑1 (MQ=255)
                      cGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc                                   <  1:1353791/71‑1 (MQ=255)
                      cGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc                                   <  1:882325/71‑1 (MQ=255)
                      cGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc                                   <  1:1269186/71‑1 (MQ=255)
                      cGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc                                   <  1:332157/71‑1 (MQ=255)
                      cGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc                                   <  1:1171160/71‑1 (MQ=255)
                      cGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc                                   <  1:2327349/71‑1 (MQ=255)
                      cGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc                                   <  1:368187/71‑1 (MQ=255)
                      cGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc                                   <  1:2203628/71‑1 (MQ=255)
                       gTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCt                                                          <  1:1640345/47‑1 (MQ=255)
                                    aaTACGTGTCATATACTTGGACCGCACGCGTTCTGCt                                                       <  1:2750138/37‑1 (MQ=38)
                                    aaTACGCGTCATATACTTGGACCGCACGCGTTCTGCt                                                       <  1:1502162/37‑1 (MQ=255)
                                    aaTACGCGTCATATACTTGGACCGCACGCGTTCTGCt                                                       <  1:1613435/37‑1 (MQ=255)
                                    aaTACGCGTCATATACTTGGACCGCACGCGTTCTGCt                                                       <  1:2912529/37‑1 (MQ=255)
                                    aaTACGCGTCATATACTTGGACCGCACGCGTTCTGCt                                                       <  1:1400644/37‑1 (MQ=255)
                                    aaTACGCGTCATATACTTGGACCGCACGCGTTCTGCt                                                       <  1:31810/37‑1 (MQ=255)
                                    aaTACGCGTCATATACTTGGACCGCACGCGTTCTGCt                                                       <  1:1155838/37‑1 (MQ=255)
                                    aaTACGCGTCATATACTTGGACCGCACGCGTTCTGCt                                                       <  1:409833/37‑1 (MQ=255)
                                     aTACGCGTCATATACTTGGACCGCACGCGTTCTGCt                                                       <  1:2276888/36‑1 (MQ=255)
                                      tACGCGTCATATACTTGGACCGCACGCGTTCTGCTTc                                                     >  1:2075244/1‑37 (MQ=255)
                                      tACGCGTCATATACTTGGACCGCACGCGTTCTGCTTc                                                     >  1:2215329/1‑37 (MQ=255)
                                      tACGCGTCATATACTTGGACCGCACGCGTTCTGCTTc                                                     >  1:3268600/1‑37 (MQ=255)
                                      tACGCGTCATATACTTGGACCGCACGCGTTCTGCTTc                                                     >  1:3072672/1‑37 (MQ=255)
                                      tACGCGTCATATACTTGGACCGCACGCGTTCTGCTTc                                                     >  1:1958691/1‑37 (MQ=255)
                                      tACGCGTCATATACTTGGACCGCACGCGTTCTGCTTc                                                     >  1:2991347/1‑37 (MQ=255)
                                      tACGCGTCATATACTTGGACCGCACGCGTTCTGCTTc                                                     >  1:1842749/1‑37 (MQ=255)
                                      tACGCGTCATATACTTGGACCGCACGCGTTCTGCTTc                                                     >  1:1884111/1‑37 (MQ=255)
                                      tACGCGTCATATACTTGGACCGCACGCGTTCTGCTTc                                                     >  1:2572796/1‑37 (MQ=255)
                                            tCATATACTTGGACCGCACGCGTTCTGCTTCTGTc                                                 <  1:2137754/35‑1 (MQ=255)
                                            tCATATACTTGGACCGCACGCGTTCTGCTTCTGTc                                                 <  1:921590/35‑1 (MQ=255)
                                            tCATATACTTGGACCGCACGCGTTCTGCTTCTGTc                                                 <  1:519002/35‑1 (MQ=255)
                                            tCATATACTTGGACCGCACGCGTTCTGCTTCTGTc                                                 <  1:1302407/35‑1 (MQ=255)
                                            tCATATACTTGGACCGCACGCGTTCTGCTTCTGTc                                                 <  1:173467/35‑1 (MQ=255)
                                            tCATATACTTGGACCGCACGCGTTCTGCTTCTGTc                                                 <  1:1861324/35‑1 (MQ=255)
                                            tCATATACTTGGACCGCACGCGTTCTGCTTCTGTc                                                 <  1:1998051/35‑1 (MQ=255)
                                            tCATATACTTGGACCGCACGCGTTCTGCTTCTGTc                                                 <  1:224165/35‑1 (MQ=255)
                                            tCATATACTTGGACCGCACGCGTTCTGCTTCTGTc                                                 <  1:2281162/35‑1 (MQ=255)
                                            tCATATACTTGGACCGCACGCGTTCTGCTTCTGTc                                                 <  1:1926263/35‑1 (MQ=255)
                                            tCATATACTTGGACCGCACGCGTTCTGCTTCTGTc                                                 <  1:817597/35‑1 (MQ=255)
                                                 taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGttt        >  1:2460722/1‑71 (MQ=255)
                                                 taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGttt        >  1:1448099/1‑71 (MQ=255)
                                                 taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGtt         >  1:1006692/1‑70 (MQ=255)
                                                 taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGtt         >  1:1538497/1‑70 (MQ=255)
                                                 taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGtt         >  1:573396/1‑70 (MQ=255)
                                                 taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGtt         >  1:2389969/1‑70 (MQ=255)
                                                 taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGtt         >  1:2324144/1‑70 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTTTCACTGGCTCACGTTCGCAACGGCATACTGCTACGAAGAGTTTctct    >  1:254886/1‑71 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct    >  1:1432724/1‑71 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct    >  1:2996440/1‑71 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct    >  1:2982577/1‑71 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct    >  1:2974326/1‑71 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct    >  1:1021663/1‑71 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct    >  1:2895606/1‑71 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct    >  1:1678152/1‑71 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct    >  1:631445/1‑71 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct    >  1:2809458/1‑71 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct    >  1:2797236/1‑71 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct    >  1:1763336/1‑71 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct    >  1:1891257/1‑71 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct    >  1:186573/1‑71 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct    >  1:2584639/1‑71 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct    >  1:2471282/1‑71 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTActct    >  1:259771/1‑71 (MQ=255)
                                                     tGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACAGCTACGAAGAGTTTctct    >  1:253073/1‑71 (MQ=255)
                                                       gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctctac  <  1:1202209/71‑3 (MQ=255)
                                                       gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc  <  1:894385/71‑1 (MQ=255)
                                                       gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc  <  1:936438/71‑1 (MQ=255)
                                                       gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc  <  1:742501/71‑1 (MQ=255)
                                                       gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc  <  1:2935271/71‑1 (MQ=255)
                                                       gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc  <  1:2819044/71‑1 (MQ=255)
                                                       gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc  <  1:2712461/71‑1 (MQ=255)
                                                       gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc  <  1:2678027/71‑1 (MQ=255)
                                                       gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc  <  1:2536949/71‑1 (MQ=255)
                                                       gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc  <  1:243545/71‑1 (MQ=255)
                                                       gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc  <  1:1542156/71‑1 (MQ=255)
                                                       gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc  <  1:1490728/71‑1 (MQ=255)
                                                       gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc  <  1:1336598/71‑1 (MQ=255)
                                                       gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc  <  1:1289753/71‑1 (MQ=255)
                                                       gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc  <  1:1084457/71‑1 (MQ=255)
                                                       gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc  <  1:1033433/71‑1 (MQ=255)
                                                        aCCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc  <  1:3228435/70‑1 (MQ=255)
                                                           |                                                                  
ACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTC  >  minE/2450239‑2450364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑