Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,470,047	T→C	R343R (CGT→CGC)	atpD →	F1 sector of membrane‑bound ATP synthase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,470,047	0	T	C	100.0%	16.9 / NA	6	R343R (CGT→CGC)	atpD	F1 sector of membrane‑bound ATP synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/0);  total (6/0)

GTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCTGGTGGTTGGTCAGGAACACTAC  >  minE/2469979‑2470083                                                                     |                                     gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt                                      >  1:3027385/1‑69 (MQ=255) gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt                                      >  1:2748464/1‑69 (MQ=255) gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt                                      >  1:3148945/1‑69 (MQ=255) gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt                                      >  1:1549493/1‑69 (MQ=255) gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt                                      >  1:1765114/1‑69 (MQ=255) gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt                                      >  1:1829251/1‑69 (MQ=255) gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt                                      >  1:1831926/1‑69 (MQ=255) gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt                                      >  1:2126252/1‑69 (MQ=255) gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt                                      >  1:2296772/1‑69 (MQ=255) gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt                                      >  1:2376092/1‑69 (MQ=255) gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt                                      >  1:2410793/1‑69 (MQ=255) gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt                                      >  1:2491359/1‑69 (MQ=255) gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt                                      >  1:261917/1‑69 (MQ=255) gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACACGCTGGACCCCACCAGCCGt                                      >  1:2819438/1‑69 (MQ=255) gTACTGAGCCGTCAGATCGCGTCTCCGGGTATCTACCCGGCCGTTGCCCCGCTGGACTCCACCAGCCGt                                      >  1:759475/1‑69 (MQ=255)                                   aCCCTGCGGTCGACCCACTCGATTCGACCTCGCGCCAGCTGGACCCGCTGGTCGTCGGCGAAGAGCACTAc  >  1:1344601/1‑71 (MQ=255)                                   aCCCTGCGGTCGACCCACTCGATTCGACCTCGCGCCAGCTGGACCCGCTGGTCGTCGGCGAAGAGCACTAc  >  1:860584/1‑71 (MQ=255)                                   aCCCTGCGGTCGACCCACTCGATTCGACCTCGCGCCAGCTGGACCCGCTGGTCGTCGGCGAAGAGCACTAc  >  1:2766340/1‑71 (MQ=255)                                   aCCCTGCGGTCGACCCACTCGATTCGACCTCGCGCCAGCTGGACCCGCTGGTCGTCGGCGAAGAGCACTAc  >  1:1537867/1‑71 (MQ=255)                                   aCCCTGCGGTCGACCCACTCGATTCGACCTCGCGCCAGCTGGACCCGCTGGTCGTCGGCGAAGAGCACTAc  >  1:1387105/1‑71 (MQ=255)                                   aCCCTGCGGTCGACCCACTCGATTCGACCTCGCGCCAGCTGGACCCGCTGGTCGTCGGCGAAGAGCACTAc  >  1:1352157/1‑71 (MQ=255)                                                                     |                                     GTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCTGGTGGTTGGTCAGGAACACTAC  >  minE/2469979‑2470083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑