Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,473,718	C→T	F331F (TTC→TTT)	glmS →	L‑glutamine:D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,473,718	0	C	T	100.0%	101.4 / NA	32	F331F (TTC→TTT)	glmS	L‑glutamine:D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/32);  total (0/32)

CGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACCG  >  minE/2473697‑2473785                      |                                                                    cGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt                    <  1:1533459/71‑1 (MQ=255) cGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt                    <  1:2314594/71‑1 (MQ=255) cGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt                    <  1:2102599/71‑1 (MQ=255) cGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt                    <  1:1785187/71‑1 (MQ=255) cGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt                    <  1:288823/71‑1 (MQ=255) cGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt                    <  1:1569381/71‑1 (MQ=255) cGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt                    <  1:1384922/71‑1 (MQ=255) cGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt                    <  1:2974025/71‑1 (MQ=255) cGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt                    <  1:1231185/71‑1 (MQ=255) cGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt                    <  1:3003377/71‑1 (MQ=255) cGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt                    <  1:1104229/71‑1 (MQ=255)  gTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGGCGTAACAGCCTGATGATCACCTTGt                    <  1:2236041/70‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:2905030/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:2005606/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:61221/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:2820411/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:258967/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:2533630/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:842060/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:2469119/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:888052/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:2109414/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:1023019/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:1929168/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:1646290/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:1634043/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:1458453/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:1299144/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:1145522/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:112834/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:1103148/71‑1 (MQ=255)                   aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:105383/71‑1 (MQ=255)                      |                                                                    CGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACCG  >  minE/2473697‑2473785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑