Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,632,970 A→G K421R (AAA→AGA)  livM → leucine/isoleucine/valine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,632,9700AG100.0% 389.2 / NA 102K421R (AAA→AGA) livMleucine/isoleucine/valine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/101);  total (1/101)

Only 100 of 126 total aligned reads displayed.

GATCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGCC  >  minE/2632902‑2633034
                                                                    |                                                                
gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                                                >  1:944779/1‑71 (MQ=255)
gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                                                >  1:2122653/1‑71 (MQ=255)
gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                                                >  1:2767428/1‑71 (MQ=255)
gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                                                >  1:2935374/1‑71 (MQ=255)
gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                                                >  1:260162/1‑71 (MQ=255)
gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                                                >  1:1630523/1‑71 (MQ=255)
gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                                                >  1:53403/1‑71 (MQ=255)
gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                                                >  1:798577/1‑71 (MQ=255)
gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                                                >  1:857863/1‑71 (MQ=255)
gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                                                >  1:861786/1‑71 (MQ=255)
      gCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCgagaggagag                                                           >  1:2617399/1‑70 (MQ=255)
      gCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCgagaggagag                                                           >  1:299708/1‑70 (MQ=255)
      gCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCgagaggagag                                                           >  1:890282/1‑70 (MQ=255)
      gCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCgagaggagag                                                           >  1:3174190/1‑70 (MQ=255)
      gCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCgagaggagag                                                           >  1:1576122/1‑70 (MQ=255)
      gCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCgagaggagag                                                           >  1:331542/1‑70 (MQ=255)
      gCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCgagaggagag                                                           >  1:332867/1‑70 (MQ=255)
      gCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCgagaggagag                                                           >  1:2757386/1‑70 (MQ=255)
      gCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCgagaggagag                                                           >  1:2378905/1‑70 (MQ=255)
                                      cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACg                          <  1:2811921/71‑1 (MQ=255)
                                      cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACg                          <  1:93145/71‑1 (MQ=255)
                                      cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACg                          <  1:2006384/71‑1 (MQ=255)
                                      cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACg                          <  1:2707330/71‑1 (MQ=255)
                                      cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACg                          <  1:2188812/71‑1 (MQ=255)
                                      cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACg                          <  1:2055264/71‑1 (MQ=255)
                                      cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACg                          <  1:2164250/71‑1 (MQ=255)
                                      cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACg                          <  1:2112568/71‑1 (MQ=255)
                                      cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACg                          <  1:175629/71‑1 (MQ=255)
                                      cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACg                          <  1:2552884/71‑1 (MQ=255)
                                      cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACg                          <  1:1230925/71‑1 (MQ=255)
                                      cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACg                          <  1:345383/71‑1 (MQ=255)
                                      cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACg                          <  1:388369/71‑1 (MQ=255)
                                      cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACg                          <  1:407149/71‑1 (MQ=255)
                                      cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACg                          <  1:1452294/71‑1 (MQ=255)
                                      cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACg                          <  1:519484/71‑1 (MQ=255)
                                      cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACg                          <  1:252980/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:248442/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:969340/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:2654811/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:2668766/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:2816432/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:2984881/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:3091566/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:3091983/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:462934/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:538685/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:647503/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:1904604/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:1196039/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:165262/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:1690667/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:1508938/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:1232023/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:1872039/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:1897703/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:2012434/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:2035954/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:2047102/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:2086373/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:1317783/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:2349901/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:1241222/71‑1 (MQ=255)
                                        cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:2398764/71‑1 (MQ=255)
                                         aaCTGTAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:2620679/70‑1 (MQ=255)
                                         aaCTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:2128226/70‑1 (MQ=255)
                                         aaCCGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc                        <  1:1533860/70‑1 (MQ=255)
                                              aaGCTGAAAAACGGCGCAGCTAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:1398676/70‑1 (MQ=255)
                                              aaGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:1295536/70‑1 (MQ=255)
                                              aaGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:1438267/70‑1 (MQ=255)
                                              aaGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:1454430/70‑1 (MQ=255)
                                              aaGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:401379/70‑1 (MQ=255)
                                              aaGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:1470354/70‑1 (MQ=255)
                                              aaGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:847943/70‑1 (MQ=255)
                                              aaGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:2430368/70‑1 (MQ=255)
                                              aaGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:1694970/70‑1 (MQ=255)
                                              aaGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:30815/70‑1 (MQ=255)
                                              aaGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:3052613/70‑1 (MQ=255)
                                              aaGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:1094682/70‑1 (MQ=255)
                                              aaGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:2860714/70‑1 (MQ=255)
                                              aaGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:2040928/70‑1 (MQ=255)
                                              aaGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:918928/70‑1 (MQ=255)
                                              aaGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:929213/70‑1 (MQ=255)
                                              aaGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:1043065/70‑1 (MQ=255)
                                                 cTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:2923828/67‑1 (MQ=255)
                                                        aCGGCGCAGCGAGAGGAGAGGAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:3191125/60‑1 (MQ=255)
                                                           gcgcAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat                   <  1:3225273/57‑1 (MQ=255)
                                                              cAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGcc  <  1:1304116/71‑1 (MQ=255)
                                                              cAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGcc  <  1:1474772/71‑1 (MQ=255)
                                                              cAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGcc  <  1:874136/71‑1 (MQ=255)
                                                              cAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGcc  <  1:1803263/71‑1 (MQ=255)
                                                              cAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGcc  <  1:913500/71‑1 (MQ=255)
                                                              cAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGcc  <  1:2199626/71‑1 (MQ=255)
                                                              cAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGcc  <  1:1064628/71‑1 (MQ=255)
                                                              cAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGcc  <  1:2376482/71‑1 (MQ=255)
                                                              cAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGcc  <  1:1014984/71‑1 (MQ=255)
                                                               aGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGcc  <  1:543994/70‑1 (MQ=255)
                                                               aGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGcc  <  1:840698/70‑1 (MQ=255)
                                                               aGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGcc  <  1:3215310/70‑1 (MQ=255)
                                                               aGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGcc  <  1:2605643/70‑1 (MQ=255)
                                                               aGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGcc  <  1:953698/70‑1 (MQ=255)
                                                                    |                                                                
GATCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGCC  >  minE/2632902‑2633034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑