Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,639,808 A→G T245A (ACG→GCG) 
*147Q (TAA→CAA) 
ugpQ →
yhhA ←
glycerophosphodiester phosphodiesterase, cytosolic
conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,639,8080AG100.0% 105.8 / NA 29T245A (ACG→GCG) 
*147Q (TAA→CAA) 
ugpQ
yhhA
glycerophosphodiester phosphodiesterase, cytosolic
conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/29);  total (0/29)

TGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTC  >  minE/2639780‑2639868
                            |                                                            
tGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCt                    <  1:1931178/71‑1 (MQ=255)
tGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCt                    <  1:3018651/71‑1 (MQ=255)
tGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCt                    <  1:211893/71‑1 (MQ=255)
tGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCt                    <  1:436731/71‑1 (MQ=255)
tGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCt                    <  1:1664372/71‑1 (MQ=255)
tGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCt                    <  1:486407/71‑1 (MQ=255)
              ttGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCt                    <  1:34052/57‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:455260/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:682200/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:2599178/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:393653/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:355764/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:347869/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:723276/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:3206037/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:3192228/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:917435/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:2917889/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:2514441/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:2094322/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:2085715/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:1936476/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:1889636/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:157480/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:1510304/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:1445462/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:1360808/71‑1 (MQ=255)
                  tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:1317231/71‑1 (MQ=255)
                   ccGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTc  <  1:1021309/70‑1 (MQ=255)
                            |                                                            
TGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTC  >  minE/2639780‑2639868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: