BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	204,556	A→G	intergenic (+156/‑28)	alaV → / → rrlH	tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	204,556	0	A	G	100.0%	386.6 / NA	113	intergenic (+156/‑28)	alaV/rrlH	tRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/84); total (29/84)

Only 100 of 113 total aligned reads displayed.

TTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGT  >  minE/204495‑204586

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:2967578/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:1669786/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:1942659/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:203901/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:2191503/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:2225149/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:255913/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:2575927/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:2716699/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:2343116/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:2975360/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:302748/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:3277263/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:661947/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:72542/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:777938/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:910062/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:939998/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:1323457/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:1122545/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:1234408/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:1396940/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:1271090/71‑1 (MQ=255)

ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:1261798/71‑1 (MQ=255)

   aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                       <  1:584/68‑1 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAGATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:3040888/71‑1 (MQ=11)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 >  1:2945236/1‑71 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:2077644/71‑1 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:2102175/71‑1 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:63773/71‑1 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 >  1:1021566/1‑71 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 >  1:3260455/1‑71 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 >  1:2199370/1‑71 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 >  1:1322225/1‑71 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:609447/71‑1 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 >  1:2819635/1‑71 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:2363262/71‑1 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:402108/71‑1 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:2881900/71‑1 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 >  1:2874912/1‑71 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 >  1:2619825/1‑71 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 >  1:43267/1‑71 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:126161/71‑1 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:1366206/71‑1 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:1413618/71‑1 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:1723572/71‑1 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:1783775/71‑1 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 >  1:1620272/1‑71 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:1593334/71‑1 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 >  1:1563483/1‑71 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:1022056/71‑1 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 >  1:1471980/1‑71 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:199629/71‑1 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 >  1:918886/1‑71 (MQ=255)

      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACAATGATGGATCGCAAGAAACATc                 <  1:2671227/71‑1 (MQ=11)

       acTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:2873090/70‑1 (MQ=255)

        cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt               >  1:3027433/1‑71 (MQ=255)

        cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt               >  1:1606265/1‑71 (MQ=255)

        cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt               >  1:2810949/1‑71 (MQ=255)

        cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt               >  1:3081645/1‑71 (MQ=255)

        cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt               >  1:3240321/1‑71 (MQ=255)

        cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt               >  1:170923/1‑71 (MQ=255)

        cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt               >  1:2027343/1‑71 (MQ=255)

        cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt               >  1:2016949/1‑71 (MQ=255)

        cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt               >  1:2015697/1‑71 (MQ=255)

        cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt               >  1:71721/1‑71 (MQ=255)

        cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt               >  1:1975059/1‑71 (MQ=255)

                  aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:1013883/59‑1 (MQ=255)

                     gttgttCGTGAGTCTCTCCAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:3040863/71‑1 (MQ=11)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCTCAAGAAACATCTTCGGGTTGTGAGGt  <  1:108473/71‑1 (MQ=255)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:1041780/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:792124/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:1143465/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:80632/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:505355/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:2647195/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:448217/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:404446/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:1984329/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:1460618/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:1466739/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:1549507/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:1819896/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:1852741/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:1917753/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:1962936/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:1980495/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:1151539/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:2109690/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:2345121/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:240051/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:2565962/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:2610197/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:1453908/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:2678934/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:2887740/71‑1 (MQ=21)

                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:1206989/71‑1 (MQ=21)

                        gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                 <  1:806571/53‑1 (MQ=255)

                            gTGAGTCTCTCAAATTTTCCCAACACGATGATGGATCGCAACAAACATc                 <  1:330038/49‑1 (MQ=11)

                                              cGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt  <  1:736275/46‑1 (MQ=255)

TTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGT  >  minE/204495‑204586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`