Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,681,918	A→G	R598R (CGT→CGC)	yhgF ←	predicted transcriptional accessory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,681,918	0	A	G	100.0%	109.2 / NA	30	R598R (CGT→CGC)	yhgF	predicted transcriptional accessory protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/25);  total (5/25)

GATGATGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCAT  >  minE/2681855‑2681941                                                                |                        gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:2539147/70‑1 (MQ=255) gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:401180/70‑1 (MQ=255) gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:3212725/70‑1 (MQ=255) gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:3059259/70‑1 (MQ=255) gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:708390/70‑1 (MQ=255) gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:2608888/70‑1 (MQ=255) gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:1240310/70‑1 (MQ=255) gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:1335545/70‑1 (MQ=255) gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:1608780/70‑1 (MQ=255)  atgatgTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:1089061/69‑1 (MQ=255)  atgatgTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:98449/69‑1 (MQ=255)                 gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:1577833/71‑1 (MQ=255)                 gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:334376/71‑1 (MQ=255)                 gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:3082918/71‑1 (MQ=255)                 gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:3042400/71‑1 (MQ=255)                 gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:2978857/71‑1 (MQ=255)                 gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:653786/71‑1 (MQ=255)                 gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:2644519/71‑1 (MQ=255)                 gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:722155/71‑1 (MQ=255)                 gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:1940146/71‑1 (MQ=255)                 gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:1835027/71‑1 (MQ=255)                 gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:1767083/71‑1 (MQ=255)                  tCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:819154/70‑1 (MQ=255)                      cacaCCGAATTTCTCATGAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  >  1:1132922/1‑66 (MQ=255)                      cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  >  1:2917593/1‑66 (MQ=255)                      cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  >  1:2653611/1‑66 (MQ=255)                      cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  >  1:2327544/1‑66 (MQ=255)                      cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  >  1:726032/1‑66 (MQ=255)                           cGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:172820/61‑1 (MQ=255)                              aTTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:1953987/41‑1 (MQ=255)                                                                |                        GATGATGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCAT  >  minE/2681855‑2681941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑