Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,703,672	G→A	A355T (GCC→ACC)	aroB →	3‑dehydroquinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,703,672	0	G	A	100.0%	85.3 / NA	30	A355T (GCC→ACC)	aroB	3‑dehydroquinate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/0);  total (30/0)

TGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGA  >  minE/2703632‑2703706                                         |                                   tGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaacaa       >  1:571984/1‑70 (MQ=255) tGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaacaa       >  1:2657472/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCAt                                >  1:707468/1‑40 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGa                          >  1:977671/1‑46 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAg   >  1:169135/1‑69 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAg   >  1:2062374/1‑69 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAg   >  1:2451884/1‑69 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:876426/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:744676/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:652212/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:622821/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:2619997/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:510157/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:3085409/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:3016873/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:2997967/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:284546/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:2760820/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:1336525/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:2390585/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:2341222/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:2208831/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:2154445/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:1953091/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:1951502/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:1904734/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:1522013/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:1506966/1‑70 (MQ=255)      ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:1478740/1‑70 (MQ=255)      atCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa  >  1:1560308/2‑70 (MQ=255)                                         |                                   TGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGA  >  minE/2703632‑2703706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑