Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,726,174	G→A	R303R (CGC→CGT)	yheS ←	fused predicted transporter subunits and ATP‑binding components of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,726,174	0	G	A	100.0%	197.8 / NA	60	R303R (CGC→CGT)	yheS	fused predicted transporter subunits and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/22);  total (38/22)

GCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGTCGACGTG  >  minE/2726120‑2726206                                                       |                                 gCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGACGCTAAAGCgg                   >  1:1771090/1‑70 (MQ=255) gCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCgg                   >  1:897100/1‑70 (MQ=255) gCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCgg                   >  1:775166/1‑70 (MQ=255) gCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCgg                   >  1:3050183/1‑70 (MQ=255) gCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCgg                   >  1:1526968/1‑70 (MQ=255) gCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCgg                   >  1:467871/1‑70 (MQ=255) gCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCgg                   >  1:649923/1‑70 (MQ=255)           aCTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTg          <  1:2801090/69‑1 (MQ=255)           aCTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTg          <  1:2825472/69‑1 (MQ=255)           aCTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTg          <  1:2638827/69‑1 (MQ=255)           aCTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTg          <  1:2870463/69‑1 (MQ=255)           aCTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTg          <  1:2294478/69‑1 (MQ=255)           aCTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTg          <  1:433655/69‑1 (MQ=255)           aCTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTg          <  1:571122/69‑1 (MQ=255)           aCTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTg          <  1:1732399/69‑1 (MQ=255)           aCTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTg          <  1:936612/69‑1 (MQ=255)           aCTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTg          <  1:905683/69‑1 (MQ=255)           aCTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTg          <  1:795379/69‑1 (MQ=255)           aCTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTg          <  1:1640222/69‑1 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGAc     >  1:757135/1‑70 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGAc     >  1:2391501/1‑70 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:395391/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:363226/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:327511/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:3056718/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:3015971/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:816108/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:2856230/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:284557/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:705664/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:575584/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:1148687/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:2704515/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:1008534/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:1217201/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:1261299/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:1287983/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:1365826/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:1666355/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:1708996/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:1741432/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:1794485/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:1953333/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:2057999/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:2151136/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:2261869/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:240411/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:2449312/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:2558491/1‑71 (MQ=255)               tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg    >  1:2745549/1‑71 (MQ=255)                  ccATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTg  <  1:332496/70‑1 (MQ=255)                  ccATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTg  <  1:1830800/70‑1 (MQ=255)                  ccATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTg  <  1:2225341/70‑1 (MQ=255)                  ccATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTg  <  1:2257004/70‑1 (MQ=255)                  ccATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTg  <  1:648174/70‑1 (MQ=255)                  ccATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTg  <  1:1362143/70‑1 (MQ=255)                  ccATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTg  <  1:1356240/70‑1 (MQ=255)                  ccATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTg  <  1:2589471/70‑1 (MQ=255)                  ccATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTg  <  1:2684597/70‑1 (MQ=255)                   cATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTg  <  1:553238/69‑1 (MQ=255)                                                       |                                 GCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGTCGACGTG  >  minE/2726120‑2726206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑