Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,802,519	T→A	D196V (GAT→GTT)	qor ←	quinone oxidoreductase, NADPH‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,802,519	0	T	A	100.0%	211.4 / NA	62	D196V (GAT→GTT)	qor	quinone oxidoreductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (1/61);  total (1/61)

GGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGT  >  minE/2802458‑2802586                                                              |                                                                    ggAATCGTTCACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACGGCTCGACCAGAACCTCTTCAc                                                            <  1:2402380/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:1815904/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:2052814/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:2011418/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:2138488/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:1896770/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:1888901/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:1855879/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:1854149/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:1817410/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:2410121/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:2590325/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:2656784/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:41563/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:132246/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:558270/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:575977/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:1172579/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:743343/71‑1 (MQ=255) ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACAGCTCGACCAGAACCTCTTCAc                                                            <  1:926021/71‑1 (MQ=255)                               gccgGTTATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:2073165/41‑1 (MQ=38)                               gccgGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                            <  1:1343824/41‑1 (MQ=255)                                             ttAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCtttt                            >  1:245715/1‑59 (MQ=255)                                                       gACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTg        <  1:1128845/69‑1 (MQ=255)                                                       gACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTg        <  1:253161/69‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCTACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:2920908/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:688102/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:1111627/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:300260/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:2020670/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:3036558/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:3055400/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:3055948/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:704292/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:3098598/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:614637/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:3154192/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:1323162/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:524973/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:1258403/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:1196205/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:580873/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:611747/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:2394465/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:2085224/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:2088801/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:2124072/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:2145949/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:2225558/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:2310169/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:2336972/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:2701552/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:1969838/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:1578115/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:2426530/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:2465743/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:1375585/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:2606308/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:2614067/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:2667445/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGTTAATAAACTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:1991279/71‑1 (MQ=255)                                                           aGAACCTCTTCACGATAGATAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGt  <  1:689386/71‑1 (MQ=255)                                                              |                                                                    GGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGGT  >  minE/2802458‑2802586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑