| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 363,268 | T→C | I96M (ATA→ATG) | fimZ ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 363,268 | 0 | T | C | 98.7% | 283.7 / NA | 76 | I96M (ATA→ATG) | fimZ | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (49/26); minor base A (1/0); total (50/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTT > minE/363202‑363320 | cTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGCATCg > 1:989243/1‑71 (MQ=255)cTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGCATCg > 1:2898991/1‑71 (MQ=255)cTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGCATCg > 1:2915911/1‑71 (MQ=255)cTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGCATCg > 1:2442406/1‑71 (MQ=255)cTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGCATCg > 1:3141647/1‑71 (MQ=255)cTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGCATCg > 1:3176801/1‑71 (MQ=255)cTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGCATCg > 1:3293575/1‑71 (MQ=255)cTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGCATCg > 1:424284/1‑71 (MQ=255)cTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGCATCg > 1:1843951/1‑71 (MQ=255)cTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGCATCg > 1:582931/1‑71 (MQ=255)cTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGCATCg > 1:1149874/1‑71 (MQ=255)cTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGCATCg > 1:115172/1‑71 (MQ=255)cTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGCATCg > 1:1446582/1‑71 (MQ=255)cTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGCATCg > 1:906574/1‑71 (MQ=255) aTTTACTGACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCgatgat < 1:923343/71‑1 (MQ=255) aTTTACTGACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCgatgat < 1:779457/71‑1 (MQ=255) aTTTACTGACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCgatgat < 1:1605138/71‑1 (MQ=255) aTTTACTGACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCgatgat < 1:1639565/71‑1 (MQ=255) aTTTACTGACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCgatgat < 1:2984913/71‑1 (MQ=255) aTTTACTGACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCgatgat < 1:915694/71‑1 (MQ=255) aTTTACTGACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCgatgat < 1:1434625/71‑1 (MQ=255) tttACTGACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCgatgat < 1:2572364/70‑1 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCa > 1:2988941/1‑48 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTc > 1:1491081/1‑51 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGAtt > 1:2429349/1‑56 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:3067749/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:317953/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:3054849/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:1145153/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:3068515/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:3112609/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:3143587/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:385429/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:486101/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:56955/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:868284/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:699200/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:729436/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:789519/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:1552134/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:1797303/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:1852866/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:1882219/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:1695273/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:1642694/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:2066775/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:1550859/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:2221176/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:2408929/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:1352482/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:1273937/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:1161172/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:2820325/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:2615540/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:2671050/1‑70 (MQ=255) gACAAAACCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTCATTTAGATGATAAAAAt > 1:2939994/1‑70 (MQ=255) gACAAAACCGTTAGAACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:2172319/1‑70 (MQ=255) gACAAAACAGTTAGCACCAGCTTGAATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAAt > 1:2099192/1‑70 (MQ=255) aCCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACtt < 1:996810/71‑1 (MQ=255) aCCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACtt < 1:1619904/71‑1 (MQ=255) aCCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACtt < 1:702809/71‑1 (MQ=255) aCCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACtt < 1:1024064/71‑1 (MQ=255) aCCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACtt < 1:1793685/71‑1 (MQ=255) aCCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACtt < 1:2006702/71‑1 (MQ=255) aCCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACtt < 1:3288953/71‑1 (MQ=255) aCCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACtt < 1:3286119/71‑1 (MQ=255) aCCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACtt < 1:3127115/71‑1 (MQ=255) aCCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACtt < 1:2211532/71‑1 (MQ=255) aCCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACtt < 1:3096053/71‑1 (MQ=255) aCCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACtt < 1:24143/71‑1 (MQ=255) aCCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACtt < 1:2531837/71‑1 (MQ=255) aCCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACtt < 1:2586505/71‑1 (MQ=255) aCCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACtt < 1:2957660/71‑1 (MQ=255) aCCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACtt < 1:2953559/71‑1 (MQ=255) aCCGTTAGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTACGATGATAAAAATAACACtt < 1:2210772/71‑1 (MQ=255) aGCACCAGCTTGCATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACtt < 1:1475653/65‑1 (MQ=255) | CTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTT > minE/363202‑363320 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |