| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 540,901 | Δ1 bp | coding (114/888 nt) | rhtA ← | threonine and homoserine efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 540,899 | 0 | C | . | 100.0% | 19.8 / NA | 6 | coding (116/888 nt) | rhtA | threonine and homoserine efflux system |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (0/6); total (0/6) | |||||||||||
CTTAAAGAACGCGATGAGGATCAGCGTTCCTAATGCCAGACGCAGCGCAGTGACACCCGGTGCGCCCACCAGAGGAAAAAGTGACTTAGCTAACGAGGCTCCACCCTGAATAGACGCCAT > minE/540835‑540954 | cTTAAAGAACGCGATGAGGATCAGCGTTCCTAATGCCAGACGCAGCGCAGTGACACCCGGTGCG‑CCACCag < 1:271462/71‑1 (MQ=255) gATCAGCGTTCCTAATGCCAGACGCAGCGCAGTGACACCCGGTGCG‑CCACCAGAGGAAAAAGTGACTTAg < 1:251285/70‑1 (MQ=255) cagcgcagTGACACCCGGTGCG‑CCACCAGAGGAAAAAGTGACTTAGCTAACGAGGCTCCACCCTGAATAGa < 1:93204/71‑1 (MQ=255) acacCCGGTGCG‑CCACCAGAGGAAAAAGTGACTTAGCTa < 1:135526/39‑1 (MQ=38) ggTGCG‑CCACCAGAGGAAAAAGTGACTTAGCTAACGAGGCTCCACCCTGAATAGACGCCAt < 1:133592/61‑1 (MQ=255) ggTGCG‑CCACCAGAGGAAAAAGTGACTTAGCTAACGAGGCTCCACCCTGAATAGACGCCAt < 1:156549/61‑1 (MQ=255) | CTTAAAGAACGCGATGAGGATCAGCGTTCCTAATGCCAGACGCAGCGCAGTGACACCCGGTGCGCCCACCAGAGGAAAAAGTGACTTAGCTAACGAGGCTCCACCCTGAATAGACGCCAT > minE/540835‑540954 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |