| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 975,257 | 1 | . | A | 50.0% | 6.5 / 11.3 | 8 | coding (358/375 nt) | ydeH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/4); new base A (1/3); total (1/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGT > minE/975188‑975316 | aGAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑a < 1:178996/71‑1 (MQ=255) aTCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGtt < 1:154317/58‑1 (MQ=255) aaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAGAGAAAGc < 1:32662/71‑1 (MQ=255) aaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGc < 1:161876/71‑1 (MQ=255) cATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAAcc < 1:183597/71‑1 (MQ=255) aTCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAAcc < 1:19879/70‑1 (MQ=255) ttGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGt < 1:54160/37‑1 (MQ=255) aataaatTTTGTAATCGGTTAATGCCGCAGTAaaaga < 1:149438/37‑1 (MQ=38) aaatTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGt > 1:119126/1‑69 (MQ=255) | AGAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGT > minE/975188‑975316 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |