Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,427,7761.G33.3% 2.8 / 10.1 9coding (705/1131 nt)rffEUDP‑N‑acetyl glucosamine‑2‑epimerase
Reads supporting (aligned to +/- strand):  ref base . (4/2);  new base G (0/3);  total (4/5)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGTTCTCTGACGTTCGGGTTGAGATGCACCGGATAGACAATCTGGATGTCCTGGTGC‑‑‑GTGGTGGCGATGTCTGCCAGCGCGTGGCAGATTTCTTCAAAGCCA  >  minE/2427720‑2427821
                                                         |                                               
ggTTCTCTGACGTTCGGGTTGAGATGCACCGGATAGACAATCTGGATGTCCTGGTGC‑‑‑GTGGTGGCGATGTc                                 >  1:234266/1‑71 (MQ=255)
ggTTCTCTGACGTTCGGGTTGAGATGCACCGGATAGACAATCTGGATGTCCTGGTGC‑‑‑GTGGTGGCGATGTc                                 >  1:255041/1‑71 (MQ=255)
ggTTCTCTGACGTTCGGGTTGAGATGCACCGGATAGACAATCTGGATGTCCTGGTGC‑‑‑GTGGTGGCGATGTc                                 >  1:69168/1‑71 (MQ=255)
                  ttGAGATGCACCGGATAGACAATCTGGATGTCCTGGTGC‑‑‑GTGGTGGCGATGTCTGCCAGCGCGTGGCAGAt               <  1:232411/71‑1 (MQ=255)
                  ttGAGATGCACCGGATAGACAATCTGGATGTCCTGGTGC‑‑‑GTGGTGGCGATGTCTGCCAGCGCGTGGCAGAt               <  1:235400/71‑1 (MQ=255)
                          cACCGGATAGACAATCTGGATGTCCTGGTGCGTGGTGGTGGCGATGTCTGCCAGCGCGTGGCAGATttc            <  1:122890/69‑1 (MQ=255)
                          cACCGGATAGACAATCTGGATGTCCTGGTGCGTGGTGGTGGCGATGTCTGCCAGCGCGTGGCAGATttc            <  1:243196/69‑1 (MQ=255)
                           aCCGGATAGACAATCTGGATGTCCTGGTGCGTGGTGGTGGCGATGTCTGCCAGCGCGTGGCAGATttc            <  1:126898/68‑1 (MQ=255)
                                                     gTGC‑‑‑GTGGTGGCGATGTCTGCCAGCGCGTGGCAGATTTCTTCAAAGCCa  >  1:158997/1‑49 (MQ=255)
                                                         |                                               
GGTTCTCTGACGTTCGGGTTGAGATGCACCGGATAGACAATCTGGATGTCCTGGTGC‑‑‑GTGGTGGCGATGTCTGCCAGCGCGTGGCAGATTTCTTCAAAGCCA  >  minE/2427720‑2427821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑