Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,464,1340CT50.0% ‑5.8 / 10.1 10H61Y (CAC→TAC) atpIATP synthase, membrane‑bound accesory subunit
Reads supporting (aligned to +/- strand):  ref base C (0/5);  new base T (5/0);  total (5/5)
Fisher's exact test for biased strand distribution p-value = 7.94e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTG  >  minE/2464073‑2464171
                                                             |                                     
aaTAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGgcgc                              <  1:157161/71‑1 (MQ=255)
 aTAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCa                             <  1:116253/71‑1 (MQ=255)
    aGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATa                           >  1:160386/1‑70 (MQ=255)
    aGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATa                           >  1:17966/1‑70 (MQ=255)
    aGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATa                           >  1:40802/1‑70 (MQ=255)
     gCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATaca                         <  1:185840/71‑1 (MQ=255)
                    tCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCAcc                                    <  1:113110/45‑1 (MQ=255)
                    tCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCAcc                                    <  1:98365/45‑1 (MQ=255)
                             cTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTg  >  1:118428/1‑70 (MQ=255)
                             cTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTg  >  1:222462/1‑70 (MQ=255)
                                                             |                                     
AATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTG  >  minE/2464073‑2464171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑