Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,513,416	T→C	F203S (TTT→TCT)	gltS →	glutamate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,513,416	0	T	C	88.9%	19.6 / ‑3.2	9	F203S (TTT→TCT)	gltS	glutamate transporter
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (4/4);  total (5/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCGCTATCTGGTGAAACACTCCACCACGCCGAACGGTATTCCGGATGACCAGGAAGTCCCGACGGCGTTTGAAAAGCCGGATGTGGGACGCATGATCACCTCGTTGGTGCTGATTGAAACTATCGCGC  >  minE/2513346‑2513475                                                                       |                                                            gcgcgcTATCTGGTGAAACACTCCACCACGCCGAACGGTATTCCGGATGACCAGGAAGTCCCGACGGCGtt                                                             >  1:13528/1‑71 (MQ=255)                     cTCCACCACGCCGAACGGTATTCCGGATGACCAGGAAGTCCCGACGGCGTCTGAAAAGCCGGATGTGGGAc                                         >  1:67524/1‑71 (MQ=255)                           cacGCCGAACGGTATTCCGGATGACCAGGAAGTCCCGACGGCGTCTGAAAAGCCGGAtg                                               <  1:192763/59‑1 (MQ=255)                                    cGGTATTCCGGATGACCAGGAAGTCCCGACGGCGTCTGAAAAGCCGGATGTGGGACGCATg                                    <  1:82099/61‑1 (MQ=255)                                       tATTCCGGATGACCAGGAAGTCCCGACGGCGTCTGAAAAGCCGGATGTGGGACGCATGATCACCTCGTTgg                       >  1:257822/1‑71 (MQ=255)                                         ttCCGGATGACCAGGAAGTCCCGACGGCGTCTGAAAAGCCGGATGTGGGACg                                        <  1:103552/52‑1 (MQ=255)                                         ttCCGGATGACCAGGAAGTCCCGACGGCGTCTGAAAAGCCGGATGTGGGACGCAt                                     >  1:240683/1‑55 (MQ=255)                                          tCCGGATGACCAGGAAGTCCCGACGGCGTCTGAAAAGCCGGATGTGGGACg                                        <  1:4915/51‑1 (MQ=255)                                           ccGGATGACCAGGAAGTCCCGACGGCGTCTGAAAAGCCGGATGTGGGACGCATGAt                                  >  1:79753/1‑56 (MQ=255)                                                            cccGACGGCGTTTGAAAAGCCGGATGTGGGACGCATGATCACCTCGTTGGTGCTGATTGAAACTATCgcgc  >  1:173507/1‑71 (MQ=255)                                                                       |                                                            GCGCGCTATCTGGTGAAACACTCCACCACGCCGAACGGTATTCCGGATGACCAGGAAGTCCCGACGGCGTTTGAAAAGCCGGATGTGGGACGCATGATCACCTCGTTGGTGCTGATTGAAACTATCGCGC  >  minE/2513346‑2513475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑