Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,523,040	T→C	K225K (AAA→AAG)	dinD ←	DNA‑damage‑inducible protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,523,040	0	T	C	100.0%	15.7 / NA	6	K225K (AAA→AAG)	dinD	DNA‑damage‑inducible protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/3);  total (3/3)

CCAAGTTCCTGAATGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCT  >  minE/2522975‑2523076                                                                  |                                     ccAAGTTCCTGAATGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGCTTTGa                                 <  1:174578/71‑1 (MQ=255) ccAAGTTCCTGAATGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGCTTTGa                                 <  1:266903/71‑1 (MQ=255)                   tGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGCTTTGAATTCACGCCATCCCGCtt               >  1:225811/1‑71 (MQ=255)                           cTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGCTTTGAATTCACGCCATCCCGCTTGAGttttt       >  1:124068/1‑71 (MQ=255)                                              tgtgtgGTGTTTGCTTGCTGCTTTGAATTCACGCCATCCCGCTTGAGtt          <  1:131266/49‑1 (MQ=255)                                               gtgtgGTGTTTGCTTGCTGCTTTGAATTCACGCCATCCCGCTTGAGTTTTtcttct  >  1:25106/1‑56 (MQ=255)                                                                  |                                     CCAAGTTCCTGAATGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCT  >  minE/2522975‑2523076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑