Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,912,429	C→T	A135T (GCG→ACG)	pyrI ←	aspartate carbamoyltransferase, regulatory subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,912,429	0	C	T	100.0%	10.6 / NA	5	A135T (GCG→ACG)	pyrI	aspartate carbamoyltransferase, regulatory subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/2);  total (3/2)

CGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGC  >  minE/2912365‑2912461                                                                 |                                 cGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATc                            <  1:180237/71‑1 (MQ=255)       taattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGa                                >  1:278658/1‑61 (MQ=255)              ccAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGttt                >  1:201461/1‑70 (MQ=255)               cAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTc              >  1:247942/1‑71 (MQ=255)                            tGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCaagct  <  1:229979/70‑4 (MQ=255)                                                                 |                                 CGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGC  >  minE/2912365‑2912461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑