Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,986,542	A→G	L19L (TTG→CTG)	arcA ←	DNA‑binding response regulator in two‑component regulatory system with ArcB or CpxA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,986,542	0	A	G	100.0%	15.5 / NA	6	L19L (TTG→CTG)	arcA	DNA‑binding response regulator in two‑component regulatory system with ArcB or CpxA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/1);  total (5/1)

GCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTA  >  minE/2986516‑2986608                           |                                                                   gCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCg                        >  1:30936/1‑71 (MQ=255)         cTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTg                 >  1:35242/1‑70 (MQ=255)            cGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCaa                                       >  1:173237/1‑45 (MQ=255)                aaTACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGttt          >  1:147666/1‑70 (MQ=255)                aaTACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTg         >  1:147330/1‑71 (MQ=255)                        tCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTa  <  1:168581/70‑1 (MQ=255)                           |                                                                   GCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTA  >  minE/2986516‑2986608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑