Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	558,918	T→C	P55P (CCT→CCC)	cmr →	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	558,918	0	T	C	100.0%	12.1 / NA	5	P55P (CCT→CCC)	cmr	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/1);  total (4/1)

TGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCGG  >  minE/558857‑558943                                                              |                          tGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCCACTTCGAt                   >  1:12102/1‑70 (MQ=255) tGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCCACTTCGAt                   >  1:139543/1‑70 (MQ=255)             gTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCCACTTCGATGACCGCGTATCTg      >  1:59648/1‑71 (MQ=255)                tGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCCACTTCGAt                   <  1:172940/55‑1 (MQ=255)                          ggtggAACAATATCAGGCGGGCATTGATTGGGTTCCCACTTCGATGACCGCGTATCTggcgg  >  1:53550/1‑62 (MQ=255)                                                              |                          TGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCGG  >  minE/558857‑558943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑