| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,027,446 | A→G | N134S (AAC→AGC) | malX → | fused maltose and glucose‑specific PTS enzyme IIBC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,027,446 | 0 | A | G | 100.0% | 19.2 / NA | 7 | N134S (AAC→AGC) | malX | fused maltose and glucose‑specific PTS enzyme IIBC components |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (5/2); total (5/2) | |||||||||||
CTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGT > minE/1027387‑1027510 | cTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCa > 1:18368/1‑71 (MQ=255)cTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCa > 1:52216/1‑71 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGtcgatcga > 1:202137/1‑71 (MQ=255) cACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGtcgatcga > 1:196762/1‑63 (MQ=255) gTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTcggtgcggt > 1:267591/1‑71 (MQ=255) ttCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTcggtgcggtg < 1:256129/71‑1 (MQ=255) ccAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGt < 1:89948/71‑1 (MQ=255) | CTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGT > minE/1027387‑1027510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |