Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,132,803	G→A	intergenic (+119/‑38)	arpB → / → yniD	ECK1718:JW5278+JW1710:b4494; hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,132,803	0	G	A	80.0%	15.8 / ‑0.1	10	intergenic (+119/‑38)	arpB/yniD	ECK1718:JW5278+JW1710:b4494; hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (4/4);  total (5/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCATGTGCACAACGCCACCCGACGGCTGTAATAACAAAAAATCCCTTGATGCCTGTCCCTTTTGTTACACTCCGTTATCACGCACAAGAGATATGCAGGACACTGGTAT  >  minE/1132748‑1132857                                                        |                                                       aCCATGTGCACAACGCCACCCGACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTaca                                          <  1:150066/70‑1 (MQ=255)       tgCACAACGCCACCCGACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCg                                     >  1:148219/1‑69 (MQ=255)            aaCGCCACCCGACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCAc                              >  1:124695/1‑71 (MQ=255)                      gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga                    <  1:185465/71‑1 (MQ=255)                              taataaCAAAAAATCCCTTGATGCCTGTCCCTTTTGTTACACTCCGTTATCACGCACAAGAGATATGc               <  1:176195/68‑1 (MQ=255)                              taataaCAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCAc                              <  1:205122/53‑1 (MQ=255)                                ataaCAAAAAATCCCTTGATGCCTGTCCCTTTTGTTACACTCCGTTATCACGCACAAGAGATATGCa              >  1:142962/1‑67 (MQ=255)                                   aCAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAGAGATATGCAGGACACt       >  1:161694/1‑71 (MQ=255)                                        aaaTCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAGAGATATGCAGGACACTGGTAt  >  1:72391/1‑71 (MQ=255)                                               ttGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga                    <  1:172200/46‑1 (MQ=255)                                                        |                                                       ACCATGTGCACAACGCCACCCGACGGCTGTAATAACAAAAAATCCCTTGATGCCTGTCCCTTTTGTTACACTCCGTTATCACGCACAAGAGATATGCAGGACACTGGTAT  >  minE/1132748‑1132857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑