| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,798,695 | A→G | Q239R (CAA→CGA) | proV → | glycine betaine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,798,695 | 0 | A | G | 85.7% | 11.4 / ‑2.4 | 7 | Q239R (CAA→CGA) | proV | glycine betaine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (5/1); total (5/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGAT > minE/1798637‑1798756 | tttATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCGAAATGGTGAAGt > 1:150805/1‑71 (MQ=255) tATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCGAAATGGTGAAGTgg > 1:247372/1‑71 (MQ=255) gCGACCGAATTGCCATTATGCGAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCaataat > 1:194759/1‑71 (MQ=255) gCGACCGAATTGCCATTATGCGAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCaataat > 1:87352/1‑71 (MQ=255) tGCCATTATGCGAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAAt > 1:194811/1‑70 (MQ=255) gCCATTATGCGAAATGGTGAAGTGGTACAGGTCGGCacac < 1:22747/40‑1 (MQ=255) ccATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGAt < 1:94053/71‑1 (MQ=255) | TTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGAT > minE/1798637‑1798756 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |