| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,845,199 | G→T | P266T (CCG→ACG) | cysD ← | sulfate adenylyltransferase, subunit 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,845,199 | 0 | G | T | 100.0% | 14.1 / NA | 6 | P266T (CCG→ACG) | cysD | sulfate adenylyltransferase, subunit 2 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (4/2); total (4/2) | |||||||||||
ATCACGCGGCCCTGACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCGTACGGA > minE/1845141‑1845258 | aTCACGCGGCCCTGACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGTCAgtgt < 1:205566/65‑1 (MQ=255) aCGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCcaccg > 1:121333/1‑71 (MQ=255) aGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCagcagc > 1:99597/1‑70 (MQ=255) ctctTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCg > 1:105191/1‑71 (MQ=255) ctctTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCg > 1:142825/1‑71 (MQ=255) gatgatTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCGTACGGa < 1:189820/71‑1 (MQ=255) | ATCACGCGGCCCTGACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCGTACGGA > minE/1845141‑1845258 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |