Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,935,566	A→T	intergenic (‑16/+75)	prfB ← / ← recJ	peptide chain release factor RF‑2/ssDNA exonuclease, 5' ‑‑> 3'‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,935,566	0	A	T	100.0%	10.8 / NA	5	intergenic (‑16/+75)	prfB/recJ	peptide chain release factor RF‑2/ssDNA exonuclease, 5' ‑‑> 3'‑specific
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (4/1);  total (4/1)

ATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTG  >  minE/1935519‑1935603                                                |                                      aTGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTCAAAATGCGGTGa                <  1:202800/71‑1 (MQ=255)                                  gTCTGATTTCTTTTTTTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGAt    >  1:168287/1‑50 (MQ=255)                                          tCTTTTTTTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTg  >  1:138326/1‑44 (MQ=255)                                          tCTTTTTTTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTg  >  1:167854/1‑44 (MQ=255)                                          tCTTTTTTTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTg  >  1:171693/1‑44 (MQ=255)                                                |                                      ATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTG  >  minE/1935519‑1935603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑