Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,321,698	G→A	T93I (ACC→ATC)	cdh ←	CDP‑diacylglycerol phosphotidylhydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,321,698	0	G	A	87.5%	15.8 / ‑3.2	8	T93I (ACC→ATC)	cdh	CDP‑diacylglycerol phosphotidylhydrolase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (3/4);  total (4/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGGGCCAT  >  minE/2321648‑2321764                                                   |                                                                   aaTCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTa                                                <  1:109857/71‑1 (MQ=255) aaTCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTa                                                <  1:86760/71‑1 (MQ=255)          ccTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATcaaca                                                              <  1:11224/48‑1 (MQ=255)          ccTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATcaaca                                                              <  1:187373/48‑1 (MQ=255)            tGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACaaa                                                            >  1:265928/1‑48 (MQ=255)                                          gAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTgg       >  1:54591/1‑71 (MQ=255)                                                tCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGGGCCAt  >  1:49103/1‑70 (MQ=255)                                                tCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGGGCCAt  >  1:50965/1‑70 (MQ=255)                                                   |                                                                   AATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGGGCCAT  >  minE/2321648‑2321764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑