Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,892,019 +GC intergenic (‑437/+54) gudP ← / ← ygdL predicted D‑glucarate transporter/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,892,0191.G100.0% 124.0 / NA 35intergenic (‑437/+54)gudP/ygdLpredicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (20/15);  total (20/15)
*minE1,892,0192.C100.0% 123.5 / NA 35intergenic (‑437/+54)gudP/ygdLpredicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (20/15);  total (20/15)

GCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTT  >  minE/1891954‑1892074
                                                                  ||                                                       
gCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct                                                        >  1:1137331/1‑66 (MQ=255)
gCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct                                                        >  1:255730/1‑66 (MQ=255)
gCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct                                                        >  1:2152105/1‑66 (MQ=255)
gCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct                                                        <  1:3739356/69‑4 (MQ=255)
gCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct                                                        >  1:727050/1‑66 (MQ=255)
     tGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCa                                                           >  1:815915/1‑61 (MQ=255)
     tGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCa                                                           >  1:1620817/1‑61 (MQ=255)
          gAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                            >  1:1938840/1‑71 (MQ=255)
           aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                            <  1:804929/70‑1 (MQ=255)
           aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                            <  1:3023331/70‑1 (MQ=255)
           aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                             <  1:3255126/69‑1 (MQ=255)
             aaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCggg                                         <  1:1126360/71‑1 (MQ=255)
                attcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCg                                      <  1:183917/71‑1 (MQ=255)
                  tcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTc                                       <  1:3471541/68‑1 (MQ=255)
                      ttcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGAt                                   >  1:4129528/1‑68 (MQ=255)
                       tcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa                               >  1:1179216/1‑71 (MQ=255)
                        cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                             >  1:1248281/1‑56 (MQ=255)
                         aGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGaa                                              <  1:3145675/54‑1 (MQ=255)
                          gCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGt                            <  1:921679/71‑1 (MQ=255)
                          gCACCGCTAAAACGACATTTACCGCTCGATGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGt                            <  1:3669645/71‑1 (MQ=255)
                           cACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGt                            >  1:3724948/1‑70 (MQ=255)
                             ccGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcg                         >  1:1324658/1‑71 (MQ=255)
                               tcTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc                        >  1:2738320/2‑70 (MQ=255)
                               gCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGAc                              >  1:3113126/1‑64 (MQ=255)
                               gCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc                        >  1:1334425/1‑70 (MQ=255)
                                cTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGAc                              >  1:741497/1‑63 (MQ=255)
                                 tAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtc                     >  1:578046/1‑71 (MQ=255)
                                   aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCgg                                     <  1:285868/53‑1 (MQ=255)
                                   aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtctc                   <  1:3190947/71‑1 (MQ=255)
                                   aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtctc                   <  1:3726289/71‑1 (MQ=255)
                                    aaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCa                  <  1:77663/71‑1 (MQ=255)
                                        aCATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGAc                              >  1:1337675/1‑55 (MQ=255)
                                        aCATTTACAGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTAGGATAAGACg                             >  1:995494/1‑56 (MQ=255)
                                           tttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                                >  1:358158/1‑50 (MQ=255)
                                            ttACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                            >  1:888542/1‑37 (MQ=25)
                                            ttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGa             >  1:2381871/1‑68 (MQ=255)
                                             tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGt                                        >  1:3423705/1‑40 (MQ=255)
                                                    cgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa                               >  1:2091549/1‑42 (MQ=37)
                                                     gctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGttt  <  1:1619278/70‑1 (MQ=38)
                                                     gctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGttt  <  1:1925282/70‑1 (MQ=38)
                                                        gAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc                        >  1:1638360/1‑45 (MQ=37)
                                                        gAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtc                     >  1:826723/1‑48 (MQ=37)
                                                                  ||                                                       
GCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTT  >  minE/1891954‑1892074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: