New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? minE 1218582 =95 (1.120)6 (0.080) 5/114 5.5 6.6% coding (151/2049 nt) prc carboxy‑terminal protease for penicillin‑binding protein 3
?minE 1218610 = 83 (1.080)coding (123/2049 nt) prc carboxy‑terminal protease for penicillin‑binding protein 3
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCACGCGTGCTGATCAAATTCCGGTATTAAAGGAAGAGACGCAGCATGCGACGGTAAGTGAGCGCGTAACGTCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  minE/1218656‑1218582
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgtcgcATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTG  >  minE/1218610‑1218678
                                                                                                                                                
TCACGCGTGCTGATCAAATTCCGGTATCAAAGGAAGAGACGCAGCATGCGACGGTAAGTGAGCGCGTAAC                                                                            <  1:2346349/70‑1
  ACGCGTGCTGATCAAATTCCGGTATTAAAGGAAGAGACGCAGCATGCGACGGTAAGTGAGCGCGTAACGTC                                                                         >  1:1276218/1‑71
    GCGTGCTGATCAAATTCCGGTATTAAAGGAAGAGACGCAGCATGCGACGGTAAGTGAGCGCGTAACGTCGC                                                                       >  1:1016961/1‑71
    GCGTGCTGATCAAATTCCGGTATTAAAGGAAGAGACGCAGCATGCGACGGTAAGTGAGCGCGTAACGTCGC                                                                       >  1:1782542/1‑71
    GCGTGCTGATCAAATTCCGGTATTAAAGGAAGAGACGCAGCATGCGACGGTAAGTGAGCGCGTAACGTCGC                                                                       <  1:3055307/71‑1
    GCGTGCTGATCAAATTCCGGTATTAAAGGAAGAGACGCAGCATGCGACGGTAAGTGAGCGCGTAACGTC                                                                         >  1:1471889/1‑69
    GCGTGCTGATCAAATTCCGGTATTAAAGGAAGAGACGCAGCATGCGACGGTAAGTGAGCGCGTAACGT                                                                          <  1:507303/68‑1
     CGTGCTGATCAAATTCCGGTATTAAAGGAAGAGACGCAGCATGCGACGGTAAGTGAGCGCGTAACGTCGC                                                                       >  1:124534/1‑70
            ATCAAATTCCGGTATTAAAGGAAGAGACGCAGCATGCGACGGTAAGTGAGCGCGTAACG                                                                           <  1:2367233/59‑1
                                                CGACGGTAAGTGAGCGCGTAACGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGA                                     <  1:166721/61‑1
                                                 GACGGTAAGTGAGCGCGTAACGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTT                                       <  1:3865620/58‑1
                                                  ACGGTAAGTGAGCGCGTAACGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATC                                   >  1:1856941/1‑61
                                                   CGGTAAGTGAGCGCGTAACGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGA                                     >  1:2813321/1‑58
                                                          TGAGCGCGTAACGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAAT                                         <  1:3784824/47‑1
                                                          TGAGCGCGTAACGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAAT                                         <  1:2138023/47‑1
                                                                      GTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTG                                      <  1:4030209/38‑1
                                                                      GTCGAATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGT     >  1:584459/1‑71
                                                                       TCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTC    >  1:80063/1‑71
                                                                       TCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCG          <  1:1670481/65‑1
                                                                         GCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCT   <  1:3691713/70‑1
                                                                         GCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCT   <  1:3054673/70‑1
                                                                         GCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCT   <  1:1040236/70‑1
                                                                          CATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTG  >  1:3562863/1‑70
                                                                          CATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTG  >  1:3557973/1‑70
                                                                                                                                                
TCACGCGTGCTGATCAAATTCCGGTATTAAAGGAAGAGACGCAGCATGCGACGGTAAGTGAGCGCGTAACGTCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  minE/1218656‑1218582
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgtcgcATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTG  >  minE/1218610‑1218678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.