New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? minE = 266144563 (0.740)5 (0.060) 4/118 6.0 7.4% coding (1384/1599 nt) rtcR sigma 54‑dependent transcriptional regulator of rtcBA expression
?minE = 2661470 66 (0.830)coding (1359/1599 nt) rtcR sigma 54‑dependent transcriptional regulator of rtcBA expression
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ACTTTGCCTGGCGGCAGATAGCGATAACGTGTTCCAGTTGCATACGGTCGAAGAGATCGATGTTTTCTGCCTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  minE/2661373‑2661445
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cctcCGCGCTTACGGCGTTGCTGGGCGCAGAGGCAGAAAACATCGATCTCTTCGACCGTATGCAACTGGAACA  <  minE/2661470‑2661402
                                                                                                                                              
ACTTTGCCTGGCGGCAGATAGCGATAACGTGTTCCAGTTGCATACGGTCGAAGAGATCGATGTTTTCTGCC                                                                         >  1:2518333/1‑71
  TTTGCCTGGCGGCAGATAGCGATAACGTGTTCCAGTTGCATACGGTCGAAGAGATCGATGTTTTCTGCCTC                                                                       >  1:1770608/1‑71
          GCGGCAGATAGCGATAACGTGTTCCAGTTGCATACGGTCGAAGAGATCGATGTTTTCTGCCTC                                                                       >  1:219399/1‑63
                        TAACGTGTTCCAGTTGCATACGGTCGAAGAGATCGATGTTTTCTGCCTCTGCGCTTACGGCGTTGCTGGGC                                                 >  1:4122646/1‑71
                               TTCCAGTTGCATACGGTCGAAGAGATCGATGTTTTCTGCCTCCGCGCTTACGGCGTTGCTGGGCGCAGAG                                           <  1:2121388/70‑1
                               TTCCAGTTGCATACGGTCGAAGAGATCGATGTTTTCTGCCTCCGCGCTTACGGCGTTGCTGGGCGCAGAG                                           <  1:2168665/70‑1
                                                 GAAGAGATCGATGTTTTCTGCCTCCGCGCTTACGGCGTTGCTGGGCGCAGAG                                           >  1:2357083/1‑52
                                                     AGATCGATGTTTTCTGCCTCCGCGCTTACGGCGTTGCTGGTCGCAG                                             <  1:1491487/46‑1
                                                                     CCTCCGCGCTTACGGCGTTTCTGGGCGCAGAGGCAGAAAACATCGATCTCTTCGACCGTATGCAACT        <  1:742607/67‑1
                                                                     CCTCCGCGCTTACGGCGTTGCTGGGCGCAGAGGCAGAAAACATCGATCTCTTCGACCGTATGCAACTGGAA    <  1:3897708/71‑1
                                                                     CCTCCGCGCTTACGGCGTTGCTGGGCGCAGAGGCAGAAAACATCGATCTCTTCGACCGTATGCAACT        <  1:3022600/67‑1
                                                                     CCTCCGCGCTTACGGCGTTGCTGGGCGCAGAGGCAGAAAACATCGATCTCTTCGACCGTATGCAACT        <  1:3792956/67‑1
                                                                       TCCGCGCTTACGGCGTTGCTGGGCGCAGAGGCAGAAAACATCGATCTCTTCGACCGTATGCAACTGGAACA  >  1:1321161/1‑71
                                                                       TCCGCGCTTACGGCGTTGCTGGGCGCAGAGGCAGAAAACATCGATCTCTTCGACCGTATGCAACTGGAACA  >  1:543401/1‑71
                                                                       TCCGCGCTTACGGCGTTGCTGGGCGCAGAGGCAGAAAACATCGATCTCTTCGACCGTATGCAACTGG      <  1:2695862/67‑1
                                                                        CCGCGCTTACGGCGTTGCTGGGCGCAGAGGCAGAAAACATCGAT                            >  1:33610/1‑44
                                                                                                                                              
ACTTTGCCTGGCGGCAGATAGCGATAACGTGTTCCAGTTGCATACGGTCGAAGAGATCGATGTTTTCTGCCTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  minE/2661373‑2661445
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cctcCGCGCTTACGGCGTTGCTGGGCGCAGAGGCAGAAAACATCGATCTCTTCGACCGTATGCAACTGGAACA  <  minE/2661470‑2661402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.