| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 705,870 | 0 | A | G | 31.8% | 24.4 / 16.5 | 22 | intergenic (‑367/+128) | ymcC/serX | predicted outer membrane lipoprotein/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base A (10/5); new base G (4/3); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.17e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCC > minE/705846‑705938 | gctcgcCCTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAccc < 1:968711/71‑1 (MQ=21)gctcgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAcc < 1:3518527/70‑1 (MQ=35) cgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCgg > 1:715976/1‑71 (MQ=34) cccTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTc < 1:2589423/71‑1 (MQ=34) ccTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCg > 1:3717274/1‑71 (MQ=34) tGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCggg > 1:3712916/1‑71 (MQ=34) gCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCgggg > 1:3659993/1‑71 (MQ=21) gCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCggg > 1:634119/1‑70 (MQ=25) gCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCgggg > 1:3233395/1‑71 (MQ=34) cGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGc > 1:1509798/1‑71 (MQ=21) cGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGc > 1:2849505/1‑71 (MQ=34) ggCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCt < 1:3038579/70‑1 (MQ=25) gCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtc < 1:2716481/71‑1 (MQ=21) cAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtct > 1:2387221/1‑71 (MQ=34) aGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGGTtctc < 1:1552815/71‑1 (MQ=21) cccGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCAt < 1:3291537/71‑1 (MQ=34) cccGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCAt < 1:3035602/71‑1 (MQ=34) cGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATcc > 1:2768081/1‑71 (MQ=34) gCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtc > 1:3316563/1‑64 (MQ=35) cTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATcccc > 1:3969807/1‑71 (MQ=21) cTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATcccc > 1:2026112/1‑71 (MQ=34) tCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATccccc > 1:919421/1‑71 (MQ=34) | GCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCC > minE/705846‑705938 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |