| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,359,867 | 0 | G | A | 40.7% | 123.7 / 176.4 | 145 | noncoding (1228/2905 nt) | rrlA | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base G (44/42); new base A (38/21); total (82/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 148 total aligned reads displayed.
CCCGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAG > minE/2359815‑2359937 | cccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTca < 1:2478658/71‑1 (MQ=31) ccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTcac < 1:1907876/71‑1 (MQ=255) ccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca < 1:1620062/70‑1 (MQ=255) gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAg < 1:2799074/71‑1 (MQ=34) gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAg > 1:1090825/1‑71 (MQ=255) cTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAgg > 1:1831136/1‑71 (MQ=34) ttATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGc < 1:2497362/70‑1 (MQ=255) tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt > 1:745430/1‑70 (MQ=35) tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGc < 1:1571054/69‑1 (MQ=255) aTCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCtt < 1:3415723/70‑1 (MQ=35) tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa > 1:2760540/1‑70 (MQ=35) tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa < 1:2927051/70‑1 (MQ=35) cGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGc > 1:1099062/1‑66 (MQ=255) cGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGc > 1:1651392/1‑66 (MQ=255) gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg < 1:82701/71‑1 (MQ=34) gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg < 1:116823/71‑1 (MQ=34) gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAg < 1:4032489/71‑1 (MQ=255) gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAg < 1:196342/71‑1 (MQ=255) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcacac > 1:1192047/1‑59 (MQ=25) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt > 1:403929/1‑63 (MQ=35) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAAc > 1:1084273/1‑71 (MQ=255) cTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGaa < 1:476668/69‑1 (MQ=255) cTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACg < 1:3328497/71‑1 (MQ=255) ttATGTCAGCATTCGCACTTCTGATACCTCCAGCCTGCCTCACAGCACACCTTCACAGGCTTACAGAACGc > 1:4021370/1‑71 (MQ=21) ttATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcac > 1:4044564/1‑56 (MQ=33) tATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTca > 1:1536210/1‑54 (MQ=31) aTGTCAGGATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCt < 1:3931598/70‑1 (MQ=25) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGa > 1:523256/1‑65 (MQ=35) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGc < 1:2124374/69‑1 (MQ=35) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCt < 1:855020/70‑1 (MQ=35) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCt < 1:3582192/70‑1 (MQ=35) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc > 1:3611693/1‑71 (MQ=255) tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa < 1:1342335/58‑1 (MQ=35) tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcccc < 1:1645560/71‑1 (MQ=34) tCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcccc > 1:2681728/1‑71 (MQ=255) tCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTccc < 1:3502110/70‑1 (MQ=255) aGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa > 1:1762592/1‑56 (MQ=35) aGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACa > 1:857708/1‑58 (MQ=35) aGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACa > 1:2295310/1‑58 (MQ=35) aGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGc < 1:2289669/64‑1 (MQ=35) aGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGc < 1:2883708/64‑1 (MQ=35) aGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCt < 1:2733299/70‑1 (MQ=35) aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCt < 1:171095/70‑1 (MQ=255) aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTa > 1:2764927/1‑71 (MQ=255) gCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAc > 1:3262005/1‑71 (MQ=255) gCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAc > 1:786835/1‑71 (MQ=255) cATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAcc < 1:1485551/71‑1 (MQ=255) aTTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAcc > 1:3508749/1‑70 (MQ=35) aTTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAcc > 1:2658153/1‑70 (MQ=255) ttCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc > 1:4023536/1‑70 (MQ=35) ttCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc > 1:1114170/1‑70 (MQ=35) ttCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCca < 1:716107/71‑1 (MQ=34) tCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTccc > 1:3667439/1‑63 (MQ=35) tCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCca > 1:114220/1‑70 (MQ=35) cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:3348181/1‑70 (MQ=255) cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:1686594/1‑70 (MQ=255) cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:3092720/1‑70 (MQ=255) cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:602553/1‑70 (MQ=255) cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:750169/1‑70 (MQ=255) gCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaaca < 1:3865293/71‑1 (MQ=34) gCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCt < 1:492128/58‑1 (MQ=11) gCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCt < 1:1928316/58‑1 (MQ=11) gCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac > 1:1802924/1‑70 (MQ=255) cACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAg < 1:2449422/51‑1 (MQ=11) aCTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa > 1:637354/1‑70 (MQ=35) aCTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa > 1:1064268/1‑70 (MQ=35) aCTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa > 1:4099463/1‑70 (MQ=35) aCTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAccc < 1:3386574/65‑1 (MQ=255) aCTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaac > 1:1725147/1‑71 (MQ=255) aCTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaac > 1:130764/1‑71 (MQ=255) ttCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:3054375/1‑65 (MQ=255) ttCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:1330007/1‑65 (MQ=255) ttCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg > 1:312633/1‑69 (MQ=11) cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACa > 1:403679/1‑45 (MQ=38) cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGa > 1:2156168/1‑47 (MQ=38) tGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc > 1:2250442/1‑52 (MQ=11) tGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc > 1:2150280/1‑52 (MQ=11) tGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc > 1:1341663/1‑52 (MQ=11) aTACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATa < 1:20947/69‑1 (MQ=40) aTACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgt < 1:3589457/71‑1 (MQ=255) tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtt < 1:3586358/71‑2 (MQ=255) tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtg < 1:3730354/71‑1 (MQ=255) tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtg < 1:1683277/71‑1 (MQ=255) tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtg < 1:3270490/71‑1 (MQ=255) tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtg < 1:1850553/71‑1 (MQ=255) tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtg < 1:2448564/71‑1 (MQ=255) tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtg < 1:2736891/71‑1 (MQ=255) ccTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtgt < 1:814559/70‑1 (MQ=255) cTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCg < 1:366255/71‑1 (MQ=255) ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc > 1:1791073/1‑44 (MQ=38) ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt > 1:628146/1‑71 (MQ=255) ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt > 1:796459/1‑71 (MQ=255) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTg < 1:2020852/71‑1 (MQ=255) aGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg > 1:1039617/1‑56 (MQ=11) gCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGcc > 1:2208349/1‑71 (MQ=255) cATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTa > 1:2891845/1‑45 (MQ=38) cATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCg > 1:1111043/1‑71 (MQ=255) aTGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGc < 1:685003/71‑1 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAg > 1:105606/1‑71 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGACGCAg > 1:4145082/1‑71 (MQ=255) | CCCGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAG > minE/2359815‑2359937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |