| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,359,876 | 0 | C | T | 35.5% | 210.2 / 178.9 | 172 | noncoding (1219/2905 nt) | rrlA | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base C (57/54); new base T (38/23); total (95/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 175 total aligned reads displayed.
CCCGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTGC > minE/2359815‑2359946 | ccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca < 1:1620062/70‑1 (MQ=255) cGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTca > 1:327871/1‑69 (MQ=31) gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAg > 1:1900734/1‑71 (MQ=34) gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAg > 1:2858889/1‑71 (MQ=34) gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAg > 1:1090825/1‑71 (MQ=255) gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAg > 1:4038348/1‑71 (MQ=255) cTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAgg > 1:3587016/1‑71 (MQ=34) ttATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGc < 1:2497362/70‑1 (MQ=255) tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt > 1:745430/1‑70 (MQ=35) tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGc < 1:1571054/69‑1 (MQ=255) tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa < 1:2927051/70‑1 (MQ=35) tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa > 1:2760540/1‑70 (MQ=35) cGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGc > 1:1099062/1‑66 (MQ=255) gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg < 1:116823/71‑1 (MQ=34) gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg < 1:82701/71‑1 (MQ=34) gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAg < 1:4032489/71‑1 (MQ=255) gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAg < 1:196342/71‑1 (MQ=255) ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGa < 1:3519348/71‑1 (MQ=34) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcacac > 1:1192047/1‑59 (MQ=25) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAAc > 1:603351/1‑71 (MQ=34) cTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACg < 1:3328497/71‑1 (MQ=255) ttATGTCAGCATTCGCACTTCTGATACCTCCAGCCTGCCTCACAGCACACCTTCACAGGCTTACAGAACGc > 1:4021370/1‑71 (MQ=21) ttATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcac > 1:4044564/1‑56 (MQ=33) tATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTca > 1:1536210/1‑54 (MQ=31) aTGTCAGGATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCt < 1:3931598/70‑1 (MQ=25) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCt < 1:855020/70‑1 (MQ=35) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCt < 1:3582192/70‑1 (MQ=35) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc > 1:4092424/1‑71 (MQ=255) tGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCTCACCTTCACAGGCTTACAGAACGCTc < 1:1479395/70‑1 (MQ=25) tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa < 1:1342335/58‑1 (MQ=35) tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcccc < 1:1645560/71‑1 (MQ=34) tCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcccc < 1:3435879/71‑1 (MQ=11) tCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTccc < 1:3502110/70‑1 (MQ=255) tCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCAAAGGCTTACAGAACGCTcccc < 1:1738335/71‑1 (MQ=11) aGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAgg > 1:764933/1‑52 (MQ=35) aGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa > 1:1762592/1‑56 (MQ=35) aGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACa > 1:2295310/1‑58 (MQ=35) aGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCt < 1:2733299/70‑1 (MQ=35) aGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCt < 1:2281240/70‑1 (MQ=35) aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTcaca < 1:2464389/50‑1 (MQ=11) aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCt < 1:171095/70‑1 (MQ=255) aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTa > 1:90104/1‑71 (MQ=255) aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTa > 1:2628273/1‑71 (MQ=255) gCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAc > 1:3262005/1‑71 (MQ=255) aTTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAcc > 1:2658153/1‑70 (MQ=255) ttCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc > 1:4023536/1‑70 (MQ=35) ttCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc > 1:1703886/1‑70 (MQ=35) ttCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca < 1:1811722/44‑1 (MQ=11) tCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTccc > 1:3667439/1‑63 (MQ=35) tCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:42200/1‑71 (MQ=34) tCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCca > 1:114220/1‑70 (MQ=35) cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa < 1:2757374/50‑1 (MQ=38) cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:1686594/1‑70 (MQ=255) cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:3348181/1‑70 (MQ=255) cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:3092720/1‑70 (MQ=255) cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:1667549/1‑70 (MQ=255) gCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCt < 1:1928316/58‑1 (MQ=11) gCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCt < 1:492128/58‑1 (MQ=11) gCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac > 1:1802924/1‑70 (MQ=255) aCTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa > 1:2143441/1‑70 (MQ=35) aCTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAccc < 1:3386574/65‑1 (MQ=255) aCTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaac > 1:130764/1‑71 (MQ=255) ttCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:1330007/1‑65 (MQ=255) ttCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:1113721/1‑65 (MQ=255) ttCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:3054375/1‑65 (MQ=255) ttCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg > 1:312633/1‑69 (MQ=11) tCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAc > 1:2089966/1‑60 (MQ=11) cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGa > 1:2156168/1‑47 (MQ=38) cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc > 1:3625846/1‑54 (MQ=35) tGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc > 1:1341663/1‑52 (MQ=11) aTACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATa < 1:20947/69‑1 (MQ=40) aTACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgt < 1:3589457/71‑1 (MQ=255) aTACCTCCAGCATGCCTAACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAg < 1:106498/70‑1 (MQ=255) tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtg < 1:2448564/71‑1 (MQ=255) tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtg < 1:3730354/71‑1 (MQ=255) tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtg < 1:1683277/71‑1 (MQ=255) tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtg < 1:1850553/71‑1 (MQ=255) ccTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtgt < 1:814559/70‑1 (MQ=255) ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt > 1:628146/1‑71 (MQ=255) ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt > 1:796459/1‑71 (MQ=255) aGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg > 1:1039617/1‑56 (MQ=11) gCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGcc > 1:2208349/1‑71 (MQ=255) cATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTa > 1:2891845/1‑45 (MQ=38) cATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCg > 1:1111043/1‑71 (MQ=255) aTGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGc < 1:685003/71‑1 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc < 1:721250/37‑1 (MQ=40) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAg > 1:105606/1‑71 (MQ=255) cTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCt < 1:518335/39‑1 (MQ=255) cacaGCACACCTTCACATGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTc < 1:2241002/57‑1 (MQ=255) acaGCACACCTTGACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTc < 1:531259/70‑1 (MQ=255) acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCtt < 1:495486/69‑1 (MQ=255) acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCtt < 1:2792361/69‑1 (MQ=255) acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTc < 1:3826137/70‑1 (MQ=255) acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTc < 1:3475635/70‑1 (MQ=255) acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCg > 1:638710/1‑71 (MQ=255) caGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCgg > 1:2897666/1‑71 (MQ=255) aGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGt > 1:2414474/1‑71 (MQ=255) gCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTg > 1:4123952/1‑71 (MQ=255) cacaCCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:2683029/1‑38 (MQ=40) cacaCCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTg < 1:388386/70‑1 (MQ=255) | CCCGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTGC > minE/2359815‑2359946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |