Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	74,556	G→A	S61S (TCG→TCA)	mraZ →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	74,556	0	G	A	100.0%	266.1 / NA	85	S61S (TCG→TCA)	mraZ	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/45);  total (40/45)

GACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCG  >  minE/74488‑74620                                                                     |                                                                 gACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATcacg                                                                >  1:2490005/1‑71 (MQ=255) gACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATcacg                                                                >  1:1744637/1‑71 (MQ=255)  aCATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATcacg                                                                <  1:3552364/70‑1 (MQ=255)  aCATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGt                                                               >  1:590209/1‑71 (MQ=255)  aCATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGt                                                               <  1:1825621/71‑1 (MQ=255)    aTTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCt                                                             >  1:3692857/1‑71 (MQ=255)     tttATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTg                                                            <  1:1459096/71‑1 (MQ=255)       tATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGt                                                           <  1:898774/70‑1 (MQ=255)         tCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTc                                                          >  1:953288/1‑69 (MQ=255)         tCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCg                                                         <  1:196543/70‑1 (MQ=255)         tCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCg                                                         <  1:1759735/70‑1 (MQ=255)           aCCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCg                                                         >  1:3827644/1‑68 (MQ=255)           aCCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAg                                                       >  1:965773/1‑70 (MQ=255)           aCCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGc                                                      <  1:1339834/71‑1 (MQ=255)           aCCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGc                                                      <  1:70371/71‑1 (MQ=255)           aCCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGc                                                      <  1:108683/71‑1 (MQ=255)             ccGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGa                                                        >  1:1817093/1‑67 (MQ=255)             ccGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGa                                                        >  1:602093/1‑67 (MQ=255)             ccGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCAt                                                    <  1:2897291/71‑1 (MQ=255)               gTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATg                                                   >  1:3207138/1‑70 (MQ=255)               gTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATg                                                   >  1:1355816/1‑70 (MQ=255)                tGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGaa                                                 >  1:1525063/1‑71 (MQ=255)                 gCCTGCTGCTTTACCCCCTGCCTGAATGGCAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAAc                                                >  1:25805/1‑71 (MQ=255)                  ccTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTGGAGCATGAAcc                                               >  1:1997477/1‑71 (MQ=255)                   ctgctgCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAAccc                                              <  1:3656094/71‑1 (MQ=255)                   ctgctgCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAAccc                                              <  1:442212/71‑1 (MQ=255)                      ctgctTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCAt                                                    >  1:791060/1‑62 (MQ=255)                      ctgctTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCgg                                            <  1:503152/70‑1 (MQ=255)                       tgctTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGtt                                          >  1:3927205/1‑71 (MQ=255)                        gctTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGtt                                          >  1:1075630/1‑70 (MQ=255)                         ctTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATg                                                   <  1:123872/60‑1 (MQ=255)                         ctTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGtt                                          <  1:410511/69‑1 (MQ=255)                         ctTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGtt                                          >  1:834198/1‑69 (MQ=255)                         ctTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGtt                                          <  1:2692223/69‑1 (MQ=255)                         ctTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTg                                         <  1:3273233/70‑1 (MQ=255)                         ctTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGa                                        <  1:820421/71‑1 (MQ=255)                           ttACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAgc                                      >  1:662284/1‑71 (MQ=255)                           ttACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAgc                                      >  1:1373983/1‑71 (MQ=255)                            tACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGaa                                                 >  1:3733055/1‑59 (MQ=255)                            tACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAgcg                                     >  1:3695827/1‑71 (MQ=255)                            tACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAgc                                      <  1:1010230/70‑1 (MQ=255)                              cccccTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGcc                                   <  1:1507206/71‑1 (MQ=255)                               ccccTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACTTCTGTCGAGCATGAACCCgg                                            <  1:2740376/61‑1 (MQ=255)                               ccccTGCCTGAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGtt                                          <  1:2053632/63‑1 (MQ=255)                                 cctgcctgAATGGGAAATTATCGAGCAAAAATTATCACGTCTGt                                                           >  1:2264723/1‑44 (MQ=255)                                 cctgcctgAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCa                                                     >  1:1741930/1‑50 (MQ=255)                                 cctgcctgAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCgtg                                >  1:1947422/1‑71 (MQ=255)                                 cctgcctgAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTAGAGCGCCgtg                                >  1:1934747/1‑71 (MQ=255)                                  ctgcctgAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAAccag                                             >  1:1491532/1‑55 (MQ=255)                                  ctgcctgAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCgg                                            <  1:2365574/58‑1 (MQ=255)                                  ctgcctgAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCgg                                            <  1:2183746/58‑1 (MQ=255)                                   tgcctgAATGGGAAATTATCGAGCAAAAATTATcacg                                                                >  1:2012118/1‑37 (MQ=255)                                     cctgAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGtgtg                              >  1:928628/1‑69 (MQ=255)                                       tgAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCAAGCATGAACCCGGTTGAGCGCCGTGTGCAgc                          >  1:3906523/1‑71 (MQ=255)                                        gAATGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGa                                        <  1:3496229/56‑1 (MQ=255)                                         aaTGGGAAATTATCGAGCAAAAATTATCACGACTGTCGAGCATGAACCCg                                             >  1:318126/1‑50 (MQ=39)                                          aTGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAAccc                                              >  1:2139629/1‑48 (MQ=255)                                          aTGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAgcgc                        >  1:181570/1‑70 (MQ=255)                                           tGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGa                                                        <  1:507379/37‑1 (MQ=255)                                           tGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGa                                                        <  1:441113/37‑1 (MQ=255)                                           tGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGa                                                        <  1:1063284/37‑1 (MQ=255)                                           tGGGAAATTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCt                      <  1:252299/71‑1 (MQ=255)                                              gAAATTATCGAGAAAAAATTATCACGTCTGTCGAGCATg                                                   <  1:2415611/39‑1 (MQ=37)                                               aaaTTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGtt                                          >  1:1766183/1‑47 (MQ=255)                                                aaTTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTg                                         >  1:3629549/1‑47 (MQ=255)                                                aaTTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGt                 >  1:1255368/1‑71 (MQ=255)                                                aaTTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCAGTGTGCAGCGCCTACTGt                 <  1:1749959/71‑1 (MQ=255)                                                 aTTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGtt                <  1:89594/71‑1 (MQ=255)                                                 aTTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGt                 >  1:2096746/1‑70 (MQ=255)                                                 aTTATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGt                 >  1:2077768/1‑70 (MQ=255)                                                  ttATCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTa               <  1:1776407/71‑1 (MQ=255)                                                    aTCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAgcgc                                    >  1:3981907/1‑48 (MQ=255)                                                     tCGAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGc                             <  1:2297854/54‑1 (MQ=255)                                                       gAGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTc           <  1:2318178/70‑1 (MQ=255)                                                        aGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCAt         <  1:2402905/71‑1 (MQ=255)                                                        aGCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCAt         <  1:1832535/71‑1 (MQ=255)                                                         gCAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCAt         <  1:314527/70‑1 (MQ=255)                                                          cAAAAATTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGc       >  1:2478471/1‑71 (MQ=255)                                                           aaaaaTTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAgc                          <  1:2938558/51‑1 (MQ=255)                                                           aaaaaTTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAgc                          <  1:3633079/51‑1 (MQ=255)                                                           aaaaaTTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCAt         <  1:763810/68‑1 (MQ=255)                                                           aaaaaTTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGcc      <  1:1853400/71‑1 (MQ=255)                                                           aaaaaTTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGcc      <  1:1419760/71‑1 (MQ=255)                                                            aaaaTTATCACGTCTGTCGAGCATGAACCCGGGTGAGCGCCgtg                                <  1:1658128/44‑1 (MQ=38)                                                             aaaTTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAg    >  1:3795854/1‑71 (MQ=255)                                                               aTTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCAt         >  1:3597990/1‑64 (MQ=255)                                                               aTTATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCg  <  1:2648344/71‑1 (MQ=255)                                                                ttATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAg              >  1:3910938/1‑58 (MQ=255)                                                                ttATCACGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCg  <  1:1088018/70‑1 (MQ=255)                                                                     |                                                                 GACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCG  >  minE/74488‑74620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑