Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	474,288	T→C	V617A (GTT→GCT)	mngA →	fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzyme IIABC components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	474,288	0	T	C	100.0%	133.0 / NA	37	V617A (GTT→GCT)	mngA	fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzyme IIABC components
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/21);  total (16/21)

GACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCC  >  minE/474229‑474353                                                            |                                                                  gACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTAt                                                                <  1:3394259/63‑1 (MQ=255)  acacCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAAt                                                       <  1:3544357/71‑1 (MQ=255)      ccGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGc                                                   <  1:315567/71‑1 (MQ=255)        ggTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTg                                                 >  1:4153884/1‑71 (MQ=255)               gCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCaa                                                        >  1:2207051/1‑57 (MQ=255)                  ttttCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAAtt                                                      <  1:3448433/56‑1 (MQ=255)                    ttCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATggc                                                             >  1:160577/1‑47 (MQ=255)                    ttCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATggc                                                             >  1:3268994/1‑47 (MQ=255)                    ttCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCgg                                      <  1:2071584/70‑1 (MQ=255)                     tctcGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCa                                    >  1:1149302/1‑71 (MQ=255)                      ctcGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCa                                    <  1:775854/70‑1 (MQ=255)                         gCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATtgg                                <  1:3619957/71‑1 (MQ=255)                         gCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATtg                                 <  1:3132946/70‑1 (MQ=255)                          ctctTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATtggt                               <  1:3582368/71‑1 (MQ=255)                          ctctTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCAGCATtggt                               <  1:3389293/71‑1 (MQ=255)                           tctTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATtggt                               <  1:4077116/70‑1 (MQ=255)                            ctTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGTCTGGTTTGGCGCgg                                      <  1:1546098/62‑1 (MQ=255)                            ctTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGtt                                              <  1:3082258/54‑1 (MQ=255)                            ctTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGtt                                              <  1:692376/54‑1 (MQ=255)                            ctTTTTACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCgg                                      <  1:202254/62‑1 (MQ=255)                               tttACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCt                                                  >  1:318858/1‑47 (MQ=255)                               tttACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTggggg                          >  1:3344142/1‑71 (MQ=255)                                 tACTTCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGc                         <  1:2433325/70‑1 (MQ=255)                                    ttCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGttt                                             <  1:319274/47‑1 (MQ=255)                                    ttCATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATtggtg                              <  1:162138/62‑1 (MQ=255)                                      cATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTgg                                           >  1:3455243/1‑47 (MQ=255)                                      cATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAAt                   >  1:3631371/1‑71 (MQ=255)                                      cATGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAAt                   >  1:2571372/1‑71 (MQ=255)                                        tGATAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAAtct                 >  1:664297/1‑71 (MQ=255)                                          aTAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAAtctc                <  1:2812021/70‑1 (MQ=255)                                           tAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGa              >  1:98055/1‑71 (MQ=255)                                           tAATGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATAGGTGGGGGCTCCAATCTCg               >  1:1658639/1‑70 (MQ=255)                                            aaTGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGAc             >  1:625031/1‑71 (MQ=255)                                             aTGGCGCGGGCGGTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACt            >  1:3358715/1‑71 (MQ=255)                                                   cgggcggTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAAt       <  1:392371/70‑1 (MQ=255)                                                    gggcggTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATtc     <  1:3591861/71‑1 (MQ=255)                                                        ggTGCTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTcc  >  1:3054695/1‑70 (MQ=255)                                                            |                                                                  GACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCC  >  minE/474229‑474353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑