Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	667,574	C→A	T304T (ACC→ACA)	uup →	fused predicted transporter subunits and ATP‑binding components of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	667,574	0	C	A	100.0%	260.9 / NA	82	T304T (ACC→ACA)	uup	fused predicted transporter subunits and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/52);  total (30/52)

AAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTT  >  minE/667507‑667638                                                                    |                                                                 aaGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGc                                                                >  1:1634390/1‑70 (MQ=255)    gCCGCGTTCGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCaaa                                                             <  1:4025882/70‑1 (MQ=255)       gcgTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATg                                                         >  1:2015199/1‑71 (MQ=255)        cgTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCACAGATGc                                                        <  1:533356/71‑1 (MQ=255)        cgTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATg                                                         >  1:3524122/1‑70 (MQ=255)        cgTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGc                                                        <  1:2694037/71‑1 (MQ=255)        cgTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGc                                                        <  1:3912530/71‑1 (MQ=255)         gTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATg                                                         >  1:1397985/1‑69 (MQ=255)         gTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGc                                                        <  1:2942154/70‑1 (MQ=255)          tACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAg                                                      >  1:2616728/1‑71 (MQ=255)          tACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAg                                                      <  1:2528198/71‑1 (MQ=255)          tACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAg                                                      <  1:2669329/71‑1 (MQ=255)             gcgcCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTg                                                   <  1:1762331/71‑1 (MQ=255)              cgcCCTGTAAGCGATGCGTCGAGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTgg                                                  <  1:3187553/71‑1 (MQ=255)                cccTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGa                                                 <  1:2222747/70‑1 (MQ=255)                 ccTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAg                                                            >  1:3906774/1‑58 (MQ=255)                 ccTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTgg                                                  >  1:4027587/1‑68 (MQ=255)                 ccTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAag                                               <  1:292678/71‑1 (MQ=255)                 ccTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAag                                               <  1:2358465/71‑1 (MQ=255)                   tGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCaa                                                              <  1:2933930/54‑1 (MQ=255)                   tGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAagag                                             >  1:3461997/1‑71 (MQ=255)                   tGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAagag                                             >  1:3492454/1‑71 (MQ=255)                     aaaGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGc                                           <  1:300649/71‑1 (MQ=255)                     aaaGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGc                                           >  1:2340962/1‑71 (MQ=255)                     aaaGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGc                                           >  1:4085233/1‑71 (MQ=255)                      aaGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGc                                           >  1:2063535/1‑70 (MQ=255)                      aaGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGc                                           >  1:3987461/1‑70 (MQ=255)                       aGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGcc                                          <  1:2510246/70‑1 (MQ=255)                         cGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAg                                        >  1:2619906/1‑70 (MQ=255)                            tGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGc                                    <  1:2292476/71‑1 (MQ=255)                            tGCGTCGAGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAag                                               <  1:3063671/60‑1 (MQ=255)                              cGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTc                                  <  1:1914224/71‑1 (MQ=255)                                tCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCa                                                       <  1:818253/48‑1 (MQ=255)                                tCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCa                                                       <  1:2463351/48‑1 (MQ=255)                                tCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCa                                                       <  1:1544635/48‑1 (MQ=255)                                tCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCg                                >  1:1560179/1‑71 (MQ=255)                                  gcgAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCaaa                                                             <  1:2456781/40‑1 (MQ=255)                                  gcgAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCgg                               >  1:2915817/1‑70 (MQ=255)                                   cgAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATg                                                         <  1:350970/43‑1 (MQ=255)                                   cgAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATg                                                         <  1:2897594/43‑1 (MQ=255)                                   cgAACGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATg                                                         <  1:1671882/43‑1 (MQ=255)                                      aCGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTgg                                                  >  1:689401/1‑47 (MQ=255)                                      aCGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTaaaa                          >  1:2557421/1‑71 (MQ=255)                                      aCGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTaaaa                          >  1:2379344/1‑71 (MQ=255)                                      aCGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTaaaa                          >  1:2211667/1‑71 (MQ=255)                                      aCGTGGTGAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTaaaa                          >  1:2833445/1‑71 (MQ=255)                                         tggtgAACGTCGCGAAGTGATGGGTACAGCAAAGATGc                                                        <  1:718053/38‑1 (MQ=255)                                         tggtgAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCg                       <  1:3096669/71‑1 (MQ=255)                                           gtgAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGt                      <  1:976366/70‑1 (MQ=255)                                           gtgAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGt                      <  1:4110259/70‑1 (MQ=255)                                            tgAACGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAagag                                             >  1:3606832/1‑46 (MQ=255)                                              aaCGTCGCGAAGTGATGGGTACAGCAAAGATGCAg                                                      >  1:2908574/1‑35 (MQ=255)                                               aCGTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCg                 <  1:1623091/71‑1 (MQ=255)                                                 gTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGaa               <  1:3760245/71‑1 (MQ=255)                                                 gTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGaa               <  1:1451344/71‑1 (MQ=255)                                                 gTCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGaa               <  1:1255956/71‑1 (MQ=255)                                                  tCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGaaa              <  1:2625576/71‑1 (MQ=255)                                                  tCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGaa               <  1:3868311/70‑1 (MQ=255)                                                  tCGCGAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGaa               <  1:3248907/70‑1 (MQ=255)                                                   cgcgAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAAt             >  1:1759275/1‑71 (MQ=255)                                                    gcgAAGTGATGGGTACAGCAAAGATGCAGGTGGaa                                                <  1:2727661/35‑1 (MQ=255)                                                    gcgAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCg                                     >  1:3896245/1‑46 (MQ=255)                                                    gcgAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAAt             <  1:2387100/70‑1 (MQ=255)                                                    gcgAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATg            >  1:942245/1‑71 (MQ=255)                                                    gcgAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATg            <  1:1527763/71‑1 (MQ=255)                                                     cgAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATgg           <  1:146415/71‑1 (MQ=255)                                                     cgAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATgg           <  1:2313032/71‑1 (MQ=255)                                                     cgAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATgg           <  1:1880567/71‑1 (MQ=255)                                                     cgAAGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATg            <  1:3334595/70‑1 (MQ=255)                                                       aaGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGaa         <  1:434437/71‑1 (MQ=255)                                                       aaGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGaa         <  1:4084967/71‑1 (MQ=255)                                                        aGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATgg           >  1:3674703/1‑68 (MQ=255)                                                        aGTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAg        <  1:1884365/71‑1 (MQ=255)                                                         gTGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGa       >  1:1117039/1‑71 (MQ=255)                                                          tGATGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGcc                                      <  1:1503235/39‑1 (MQ=255)                                                            aTGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACg     <  1:234648/70‑1 (MQ=255)                                                            aTGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACg     <  1:629570/70‑1 (MQ=255)                                                             tGGGTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATc                        >  1:2949506/1‑50 (MQ=255)                                                               ggTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCg                       <  1:2446158/49‑1 (MQ=255)                                                               ggTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGttt  <  1:4136111/70‑1 (MQ=255)                                                               ggTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGttt  <  1:1367730/70‑1 (MQ=255)                                                                gTACAGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGt    >  1:1048986/1‑67 (MQ=255)                                                                    |                                                                 AAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTT  >  minE/667507‑667638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑