Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	760,045	T→C	T160A (ACG→GCG)	ycfZ ←	predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	760,045	0	T	C	100.0%	223.4 / NA	60	T160A (ACG→GCG)	ycfZ	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/30);  total (30/30)

TGCCGGGGGGAGCATTTTCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGTCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCATTATTGATTCCCTGATACCAGAG  >  minE/759985‑760108                                                             |                                                                tgcCGGGGGGAGCATTTTCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGttt                                                        >  1:958197/1‑70 (MQ=255) tgcCGGGGGGAGCATTTTCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGttt                                                        >  1:2924369/1‑70 (MQ=255)   ccGGGGGGAGCATTTTCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGtttt                                                       <  1:660322/69‑1 (MQ=255)   ccGGGGGGAGCATTTTCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGttt                                                        <  1:1449464/68‑1 (MQ=255)   ccGGGGGGAGCATTTTCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTaa                                                     >  1:608481/1‑71 (MQ=255)    cGGGGGGAGCATTTTCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTaa                                                     >  1:632541/1‑70 (MQ=255)      gggggAGCATTTTCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTg                                                           <  1:314309/62‑1 (MQ=255)        gggAGCATTTTCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCt                                                <  1:2507262/71‑1 (MQ=255)           aGCATTTTCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCtgtg                                             >  1:3349008/1‑71 (MQ=255)            gCATTTTCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGc                                            >  1:1645641/1‑71 (MQ=255)               ttttCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCt                                                <  1:1675254/64‑1 (MQ=255)               ttttCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTg                                         >  1:4084157/1‑71 (MQ=255)                tttCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAatat                                                  >  1:3335201/1‑61 (MQ=255)                tttCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTg                                         >  1:4125090/1‑70 (MQ=255)                 ttCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGtt                                                         >  1:2478235/1‑53 (MQ=255)                  tCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTg                                         >  1:750411/1‑68 (MQ=255)                  tCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTg                                         >  1:872136/1‑68 (MQ=255)                    aTGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGaa                                    <  1:3393044/71‑1 (MQ=255)                      gCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGaa                                    >  1:1562084/1‑69 (MQ=255)                       cTGTTGTCCTGGATGCGGATAATACGTTAGAGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAg                                 >  1:276515/1‑71 (MQ=255)                         gttgtCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGtt                                                         <  1:3817844/45‑1 (MQ=255)                         gttgtCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAg                                      >  1:3749980/1‑64 (MQ=255)                         gttgtCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAg                                      >  1:1580490/1‑64 (MQ=255)                         gttgtCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAg                                      >  1:1346683/1‑64 (MQ=255)                          ttgtCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTa                                                      <  1:2738720/47‑1 (MQ=255)                          ttgtCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTa                                                      <  1:795979/47‑1 (MQ=255)                          ttgtCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCt                                           <  1:2658538/58‑1 (MQ=255)                            gtCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCtt                                          >  1:1500959/1‑57 (MQ=255)                             tCCTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAAt                            <  1:3251454/70‑1 (MQ=255)                               cTGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCa                         >  1:1655754/1‑71 (MQ=255)                               cTGGATGCGGATAATACGATAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCa                         >  1:2573002/1‑71 (MQ=255)                                tGGATGCGGATAATACGTTAGTGGCGGCGCCGATTGtttt                                                       <  1:1227692/40‑1 (MQ=255)                                  gATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCatta                      <  1:592947/71‑1 (MQ=255)                                  gATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCatta                      >  1:3993098/1‑71 (MQ=255)                                  gATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCatta                      <  1:2910645/71‑1 (MQ=255)                                  gATGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCat                        <  1:930674/69‑1 (MQ=255)                                    tGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCattatt                    <  1:1873402/71‑1 (MQ=255)                                    tGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCattatt                    <  1:3167043/71‑1 (MQ=255)                                    tGCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCattatt                    <  1:2561150/71‑1 (MQ=255)                                     gCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGcc                               <  1:3185530/59‑1 (MQ=255)                                     gCGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCattatt                    <  1:215275/70‑1 (MQ=255)                                      cGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCATTATTg                   <  1:50922/70‑1 (MQ=255)                                      cGGATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCATTATTg                   <  1:2490823/70‑1 (MQ=255)                                        gATAATACGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCATTATTGAtt                <  1:3784155/71‑1 (MQ=255)                                         ataataCGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCATTATTGATTc               >  1:2858660/1‑71 (MQ=255)                                          taataCGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGaa                                    <  1:377410/49‑1 (MQ=255)                                          taataCGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCATTATTGATTcc              >  1:134517/1‑71 (MQ=255)                                            ataCGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCATTATTGATTccc             <  1:1132361/70‑1 (MQ=255)                                            ataCGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCATTATTGATTCCCt            <  1:666122/71‑1 (MQ=255)                                             taCGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCa                              >  1:172266/1‑52 (MQ=255)                                              aCGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGa                                     >  1:1477813/1‑44 (MQ=255)                                               cGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCATTATTGATTCCCTGa          >  1:136817/1‑70 (MQ=255)                                               cGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCATTATTGATTCCCTGa          >  1:1932762/1‑70 (MQ=255)                                               cGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCATTATTGATTCCCTGa          >  1:1012389/1‑70 (MQ=255)                                               cGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCATTATTGATTCCCTGAt         <  1:3720448/71‑1 (MQ=255)                                               cGTTAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCATTATTGATTCCCTGAt         <  1:2730257/71‑1 (MQ=255)                                                 ttAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGaa                                    >  1:1560501/1‑42 (MQ=255)                                                 ttAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGaa                                    <  1:2378243/42‑1 (MQ=255)                                                  tAGTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCATTATTGATTCCCTGATAc       >  1:2273440/1‑70 (MQ=255)                                                    gTGGCGGCGCCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCATTATTGATTCCCTGATACCa     <  1:2984973/70‑1 (MQ=255)                                                       gccgcgcCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCATTATTGATTCCCTGATACCagag  <  1:2752235/67‑1 (MQ=255)                                                             |                                                                TGCCGGGGGGAGCATTTTCATGCTGTTGTCCTGGATGCGGATAATACGTTAGTGGCGGCGTCGATTGTTTTAATATCTGTGCTTGTAGAACAGCCAATGCATTATTGATTCCCTGATACCAGAG  >  minE/759985‑760108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑