Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	786,414	G→A	T173I (ACC→ATC)	nhaB ←	sodium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	786,414	0	G	A	100.0%	287.3 / NA	91	T173I (ACC→ATC)	nhaB	sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/47);  total (44/47)

AACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCA  >  minE/786348‑786480                                                                   |                                                                   aaCTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATAc                                                                 <  1:3595336/70‑1 (MQ=255) aaCTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACg                                                                <  1:531145/71‑1 (MQ=255) aaCTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACg                                                                <  1:773504/71‑1 (MQ=255)   cTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACgg                                                               >  1:1242422/1‑70 (MQ=255)   cTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACgg                                                               >  1:1288843/1‑70 (MQ=255)   cTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACgg                                                               >  1:3050164/1‑70 (MQ=255)      ttCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGaa                                                            <  1:2346653/70‑1 (MQ=255)       tCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATAc                                                                 <  1:1871498/64‑1 (MQ=255)       tCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGaa                                                            >  1:3575554/1‑69 (MQ=255)        ccAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAagag                                                         >  1:1239491/1‑71 (MQ=255)        ccAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAagag                                                         >  1:2513460/1‑71 (MQ=255)        ccAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAaga                                                          <  1:1189288/70‑1 (MQ=255)        ccAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAaga                                                          <  1:807891/70‑1 (MQ=255)        ccAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAaga                                                          <  1:1926998/70‑1 (MQ=255)         cAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAaga                                                          <  1:3817815/69‑1 (MQ=255)         cAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAgg                                                        <  1:72353/71‑1 (MQ=255)          aGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACgg                                                               <  1:3845638/63‑1 (MQ=255)          aGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGc                                                       >  1:2101925/1‑71 (MQ=255)          aGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGc                                                       >  1:236296/1‑71 (MQ=255)           gAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATa                                                                  <  1:2889455/59‑1 (MQ=255)              ccACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTAcg                                                   <  1:2556033/71‑1 (MQ=255)              ccACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTAcg                                                   >  1:3947362/1‑71 (MQ=255)              ccACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTAcg                                                   <  1:434185/71‑1 (MQ=255)              ccACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTAcg                                                   <  1:714067/71‑1 (MQ=255)               cACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGGCGGTGGCTTCGATACGGGAAGAGGCTAcgc                                                  <  1:2101926/71‑1 (MQ=255)                   ttGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATAc                                                                 <  1:2035373/52‑1 (MQ=255)                   ttGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGt                                               >  1:1278187/1‑70 (MQ=255)                   ttGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGt                                               >  1:3337690/1‑70 (MQ=255)                      tAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGAt                                            <  1:305480/70‑1 (MQ=255)                      tAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGAt                                            <  1:25472/70‑1 (MQ=255)                      tAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATa                                           <  1:3128285/71‑1 (MQ=255)                       aaTGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATa                                           >  1:2689002/1‑70 (MQ=255)                        aTGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATaaa                                         <  1:474564/71‑1 (MQ=255)                         tGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTAcg                                                   <  1:456918/60‑1 (MQ=255)                         tGCTTGTCGATATGACTATCGTCTTGCAGGGCGGTGTCTTCGATACGGGAAGAGGCTAcg                                                   <  1:1291612/60‑1 (MQ=255)                           cTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATAc                                                                 >  1:3470792/1‑44 (MQ=255)                           cTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATAc                                                                 >  1:3118402/1‑44 (MQ=255)                           cTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTa                                                     >  1:857731/1‑56 (MQ=255)                           cTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTa                                                     >  1:677005/1‑56 (MQ=255)                           cTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTAcgcg                                                 >  1:3135284/1‑60 (MQ=255)                           cTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTAcgcg                                                 >  1:1603622/1‑60 (MQ=255)                           cTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATAc                                      >  1:2454742/1‑71 (MQ=255)                            ttGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATAc                                      >  1:1097853/1‑70 (MQ=255)                             tGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCa                                    >  1:3476763/1‑71 (MQ=255)                               tCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATa                                  <  1:2983647/71‑1 (MQ=255)                                cGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGa                                             <  1:2857723/59‑1 (MQ=255)                                cGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGa                                             <  1:696203/59‑1 (MQ=255)                                cGATATGACTATCGTCTTGCAGGTCGGGGTCTTCGATACGGGAAGAGGCTACGCGGTGa                                             <  1:2251679/59‑1 (MQ=255)                                 gATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATaaa                                <  1:2274529/71‑1 (MQ=255)                                 gATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATaaa                                <  1:3730393/71‑1 (MQ=255)                                 gATATGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATaa                                 >  1:4149382/1‑70 (MQ=255)                                  atatGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATaaa                                >  1:336140/1‑70 (MQ=255)                                   tatGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGt                                               <  1:1384677/54‑1 (MQ=255)                                   tatGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGt                                               <  1:3074776/54‑1 (MQ=255)                                     tGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATaaa                                         >  1:127187/1‑58 (MQ=255)                                     tGACTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAAcc                            >  1:3514181/1‑71 (MQ=255)                                       aCTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGa                          <  1:224384/71‑1 (MQ=255)                                       aCTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGa                          >  1:4088675/1‑71 (MQ=255)                                       aCTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGa                          <  1:359310/71‑1 (MQ=255)                                       aCTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGa                          >  1:1916881/1‑71 (MQ=255)                                        cTATCGTCTTGCAGGTCTGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGAc                         >  1:3954065/1‑71 (MQ=255)                                        cTATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGAc                         >  1:3980651/1‑71 (MQ=255)                                         tATCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACt                        >  1:632274/1‑71 (MQ=255)                                           tCGTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATaaaaa                              >  1:1184295/1‑63 (MQ=255)                                             gTCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCa                     <  1:1142331/70‑1 (MQ=255)                                              tCTTGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATa                                           <  1:2898677/47‑1 (MQ=255)                                                ttGCAGGTCGGTGTCTTCGATACGTGAAGAGGCTACGCGGt                                               <  1:2915888/41‑1 (MQ=255)                                                ttGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGc                 >  1:223776/1‑71 (MQ=255)                                                ttGCAGGGCGGTGTCTTCGATACGGGAAGAGGCTACGCGGt                                               <  1:80708/41‑1 (MQ=255)                                                 tGCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATa                                           <  1:2490853/44‑1 (MQ=255)                                                  gCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGt                                               <  1:1002008/39‑1 (MQ=255)                                                  gCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATa                                       >  1:1735789/1‑47 (MQ=255)                                                  gCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCt                <  1:3568300/70‑1 (MQ=255)                                                  gCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCt                >  1:986188/1‑70 (MQ=255)                                                  gCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCt                <  1:134808/70‑1 (MQ=255)                                                  gCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCt                >  1:440517/1‑70 (MQ=255)                                                  gCAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCt                <  1:2903953/70‑1 (MQ=255)                                                   cAGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTg               <  1:2524727/70‑1 (MQ=255)                                                    aGGTCGGTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGAt             >  1:1275405/1‑71 (MQ=255)                                                         ggTGTCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATc            >  1:168602/1‑67 (MQ=255)                                                          gtgtCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATcaccac       <  1:1928687/71‑1 (MQ=255)                                                          gtgtCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATcaccac       >  1:3278582/1‑71 (MQ=255)                                                          gtgtCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATcaccac       >  1:3700004/1‑71 (MQ=255)                                                            gtCTTCGATACGGGAAGAGGCTACGCGGTGATAAATAcc                                     >  1:381769/1‑39 (MQ=255)                                                            gtCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCg     >  1:2166703/1‑71 (MQ=255)                                                             tCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATcacc         <  1:2388235/66‑1 (MQ=255)                                                             tCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGc    <  1:2455613/71‑1 (MQ=255)                                                             tCTTCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGc    >  1:1288117/1‑71 (MQ=255)                                                               ttCGATACGGGAAGAGGCTACGCGGTGATAAATACCATaaaa                               <  1:3452491/42‑1 (MQ=255)                                                               ttCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATcacc         >  1:1669402/1‑64 (MQ=255)                                                               ttCGATACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCa  >  1:2533128/1‑71 (MQ=255)                                                                   |                                                                   AACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCA  >  minE/786348‑786480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑