Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,036,505	G→A	A632A (GCG→GCA)	rsxC →	fused predicted 4Fe‑4S ferredoxin‑type protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,036,505	0	G	A	100.0%	174.8 / NA	64	A632A (GCG→GCA)	rsxC	fused predicted 4Fe‑4S ferredoxin‑type protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/35);  total (29/35)

GCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCC  >  minE/1036437‑1036571                                                                     |                                                                   gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg                                                                  >  1:3891055/1‑71 (MQ=21)    gAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacgc                                                                 >  1:4015061/1‑69 (MQ=25)    gAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCaa                                                               <  1:1584187/71‑1 (MQ=21)    gAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCaa                                                               <  1:4113387/71‑1 (MQ=21)       ccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt                                                            <  1:318463/71‑1 (MQ=21)       ccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCt                                                            >  1:569478/1‑71 (MQ=255)         agaagaACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTgg                                                          <  1:2411315/71‑1 (MQ=21)         agaagaACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTgg                                                          <  1:3914371/71‑1 (MQ=21)          gaagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTgg                                                          >  1:3524759/1‑70 (MQ=25)            agaaCAGGTCGCTCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTgg                                                          >  1:1895038/1‑68 (MQ=11)            agaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa                                                        >  1:1263463/1‑70 (MQ=18)             gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa                                                        >  1:2372010/1‑69 (MQ=14)                             gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcc                                      <  1:800666/71‑1 (MQ=14)                             gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcc                                      <  1:3014781/71‑1 (MQ=14)                             gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcc                                      <  1:3827117/71‑1 (MQ=14)                             gcAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcc                                      <  1:2798305/71‑1 (MQ=9)                                aacccgccgTCGAAGCCGCTATAGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGa                                    <  1:3058764/67‑1 (MQ=11)                                aaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg                                   >  1:4069989/1‑71 (MQ=25)                                 aGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGc                                  <  1:3856171/71‑1 (MQ=255)                                        gTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaaca                           <  1:3315746/71‑1 (MQ=255)                                        gTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac                            >  1:1419944/1‑70 (MQ=255)                                         tCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          <  1:3149799/71‑1 (MQ=255)                                         tCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          <  1:2883382/71‑1 (MQ=255)                                         tCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          >  1:3417488/1‑71 (MQ=255)                                           gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          >  1:3861095/1‑69 (MQ=255)                                           gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          >  1:945118/1‑69 (MQ=255)                                            aaGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAg                       >  1:2717589/1‑71 (MQ=255)                                             aGCCGCTATTGCCCGTGCCAAAGCACGGAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg                      >  1:3436423/1‑71 (MQ=255)                                             aGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCa                                >  1:3754816/1‑61 (MQ=255)                                             aGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg                      <  1:2003081/71‑1 (MQ=255)                                             aGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg                      >  1:1628744/1‑71 (MQ=255)                                             aGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg                      >  1:3590827/1‑71 (MQ=255)                                              gCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGt                     <  1:740538/71‑1 (MQ=255)                                               ccGCTATTGCCCGTGCC‑AAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCg                   <  1:2327142/71‑1 (MQ=14)                                                cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTc                    >  1:2082326/1‑70 (MQ=255)                                                cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCg                   <  1:2409734/71‑1 (MQ=255)                                                 gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAacaaca                        >  1:3144679/1‑65 (MQ=255)                                                 gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa                  <  1:3991115/71‑1 (MQ=255)                                                 gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa                  <  1:408050/71‑1 (MQ=255)                                                  cTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt                 >  1:91131/1‑71 (MQ=255)                                                     ttGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcg              >  1:1417463/1‑71 (MQ=255)                                                         ccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTc                    <  1:3133351/61‑1 (MQ=255)                                                         ccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTc                    <  1:1919653/61‑1 (MQ=255)                                                         ccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTc                    <  1:2202512/61‑1 (MQ=255)                                                          cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaa         <  1:2921168/71‑1 (MQ=255)                                                           gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCa          >  1:339621/1‑69 (MQ=255)                                                            tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCgcgc           <  1:3817606/67‑1 (MQ=255)                                                            tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCgcgc           <  1:800388/67‑1 (MQ=255)                                                             gCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa                  >  1:1866179/1‑59 (MQ=255)                                                             gCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgc      >  1:2386494/1‑71 (MQ=255)                                                              ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa                              <  1:2965593/46‑1 (MQ=255)                                                              ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa                              <  1:2562073/46‑1 (MQ=255)                                                              ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa                              <  1:1474822/46‑1 (MQ=255)                                                              ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCgcgc           <  1:3491404/65‑1 (MQ=255)                                                              ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgcc     <  1:65896/71‑1 (MQ=255)                                                               cAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaa         <  1:2405776/66‑1 (MQ=255)                                                               cAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCa          <  1:1084136/65‑1 (MQ=255)                                                                aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg                      <  1:1002479/52‑1 (MQ=255)                                                                aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaa         >  1:2803308/1‑65 (MQ=255)                                                                 aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc  <  1:4032484/71‑1 (MQ=255)                                                                 aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc  >  1:2035639/1‑71 (MQ=255)                                                                 aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc  >  1:2115805/1‑71 (MQ=255)                                                                 aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc  >  1:3401076/1‑71 (MQ=255)                                                                  aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa                  >  1:4077579/1‑54 (MQ=255)                                                                  aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa                  >  1:1590361/1‑54 (MQ=255)                                                                  aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa                  >  1:2211416/1‑54 (MQ=255)                                                                     |                                                                   GCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCC  >  minE/1036437‑1036571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑