Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,312,925	G→A	A84T (GCG→ACG)	hisC →	histidinol‑phosphate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,312,925	0	G	A	100.0%	172.2 / NA	55	A84T (GCG→ACG)	hisC	histidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/32);  total (23/32)

AAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTGCGAACCGGGTAAAGACGCCATCCTC  >  minE/1312857‑1312987                                                                     |                                                               aaGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGcacg                                                              <  1:3487618/71‑1 (MQ=255)      gTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGa                                                         <  1:3924458/71‑1 (MQ=255)      gTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGGCAGCCGTGGCACGGACGa                                                         <  1:2024551/71‑1 (MQ=255)      gTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCAGGTCAGCCGTGGCACGGACGa                                                         <  1:2700158/71‑1 (MQ=255)       tGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGaa                                                        <  1:3689358/71‑1 (MQ=255)         aTTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAgg                                                      >  1:2425143/1‑71 (MQ=255)            gAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTAt                                                   >  1:1381642/1‑71 (MQ=255)             aaaaTTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTAt                                                   <  1:2306054/70‑1 (MQ=255)             aaaaTTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTAt                                                   <  1:2305744/70‑1 (MQ=255)             aaaaTTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTAt                                                   <  1:2301387/70‑1 (MQ=255)              aaaTTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTg                                                 <  1:2731661/71‑1 (MQ=255)               aaTTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACg                                                          <  1:3496284/61‑1 (MQ=255)                aTTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAg                                                       >  1:1149105/1‑63 (MQ=255)                   atgcgcAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAAct                                             <  1:893373/68‑1 (MQ=255)                    cgcgCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAActg                                            <  1:334632/70‑1 (MQ=255)                       gcAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGa                                        <  1:194973/71‑1 (MQ=255)                        cAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAActgc                                           >  1:633285/1‑67 (MQ=255)                        cAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGAt                                       <  1:2857628/71‑1 (MQ=255)                          atatGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTc                                     <  1:110960/71‑1 (MQ=255)                           tatGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTg                                                 <  1:4130284/58‑1 (MQ=255)                            atGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTcgc                                   >  1:3114555/1‑71 (MQ=255)                            atGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTcgc                                   >  1:2416851/1‑71 (MQ=255)                             tGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGaa                                               >  1:2613224/1‑58 (MQ=255)                             tGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTcgcg                                  >  1:1829214/1‑71 (MQ=255)                               cAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAAc                                              >  1:3592436/1‑57 (MQ=255)                               cAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAAc                                              >  1:1465403/1‑57 (MQ=255)                               cAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAAc                                              >  1:487793/1‑57 (MQ=255)                               cAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCAGATTCGCGCt                                >  1:703492/1‑71 (MQ=255)                                 ggCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCttt                              >  1:1993839/1‑71 (MQ=255)                                 ggCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCttt                              <  1:379909/71‑1 (MQ=255)                                  gCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCtttt                             >  1:1828313/1‑71 (MQ=255)                                   cGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGTCACGGACGAAGGTATTGAACTGCTGACTCGCGCttttt                            <  1:3740489/71‑1 (MQ=255)                                   cGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCttttt                            <  1:229353/71‑1 (MQ=255)                                    gTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTcgc                                   <  1:3940038/63‑1 (MQ=255)                                    gTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTg                           >  1:2120449/1‑71 (MQ=255)                                     tAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCtt                               <  1:901043/66‑1 (MQ=255)                                      aaaaCCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTGc                          >  1:892212/1‑70 (MQ=255)                                       aaaCCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCtttt                             >  1:2798127/1‑66 (MQ=255)                                       aaaCCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTg                           >  1:2946553/1‑68 (MQ=255)                                       aaaCCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTGCg                         <  1:3447702/70‑1 (MQ=255)                                        aaCCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTGCGaa                       <  1:781044/71‑1 (MQ=255)                                         aCCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTGCGAAc                      <  1:2996271/71‑1 (MQ=255)                                         aCCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTGCGAAc                      <  1:3956417/71‑1 (MQ=255)                                         aCCGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTGCGAAc                      <  1:3960900/71‑1 (MQ=255)                                          ccGGAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTAtt                                                  <  1:30884/42‑1 (MQ=255)                                             gAGCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTc                                     <  1:2405784/52‑1 (MQ=255)                                               gCAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTGCGAACCggg                  >  1:1551358/1‑69 (MQ=255)                                                cAGGTGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTGCGAACCGGGTaa               <  1:1485699/71‑1 (MQ=255)                                                    tGCTGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTGCGAAc                      <  1:2772028/60‑1 (MQ=255)                                                       tGGTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCAGTTTGCGAACCGGGTAAAGACg          >  1:916337/1‑69 (MQ=255)                                                         gTCAGCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTGCGAACCGGGTAAAg             <  1:3150954/64‑1 (MQ=255)                                                             gCCGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTGCGAACCGGGTAAAGACGCCATCctc  <  1:2630441/71‑1 (MQ=255)                                                              ccGTGGCACTGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTGCGAACCGCGTAAAGACGCCATCctc  >  1:3752834/1‑70 (MQ=255)                                                              ccGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTGCGAACCGGGTAAAGACGCCATCctc  >  1:20235/1‑70 (MQ=255)                                                              ccGTGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTGCGAACCGGGTAAAGACGCCATCctc  >  1:546611/1‑70 (MQ=255)                                                                 tGGCACGGACGAAGGTATTGAACTGCTGATTCGCGCttt                              <  1:1762850/39‑1 (MQ=255)                                                                     |                                                               AAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGCTGATTCGCGCTTTTTGCGAACCGGGTAAAGACGCCATCCTC  >  minE/1312857‑1312987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑