Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,348,802 C→A A473S (GCG→TCG)  asmA ← predicted assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,348,8020CA100.0% 432.5 / NA 137A473S (GCG→TCG) asmApredicted assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (70/67);  total (70/67)

Only 100 of 137 total aligned reads displayed.

GCGGTCAAGCCGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGT  >  minE/1348734‑1348869
                                                                    |                                                                   
gCGGTCAAGCCGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGAtt                                                                   >  1:542102/1‑71 (MQ=255)
 cGGTCAAGCCGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTg                                                                  >  1:22305/1‑71 (MQ=255)
    tCAAGCCGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCt                                                                >  1:3422462/1‑70 (MQ=255)
    tCAAGCCGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTg                                                               <  1:3094532/71‑1 (MQ=255)
      aaGCCGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGaa                                                             <  1:2062013/71‑1 (MQ=255)
      aaGCCGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGaa                                                             >  1:3576242/1‑71 (MQ=255)
      aaGCCGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGaa                                                             >  1:3640308/1‑71 (MQ=255)
      aaGCCGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGaa                                                             >  1:4018746/1‑71 (MQ=255)
      aaGCCGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGa                                                              <  1:1237746/70‑1 (MQ=255)
        gCCGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAatc                                                           <  1:3878229/71‑1 (MQ=255)
         ccGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAatca                                                          <  1:1067259/71‑1 (MQ=255)
          cgcgTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAatcat                                                         <  1:1361150/71‑1 (MQ=255)
          cgcgTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAatcat                                                         >  1:688271/1‑71 (MQ=255)
           gcgTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAatcatc                                                        >  1:173195/1‑71 (MQ=255)
             gTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATct                                                       <  1:2544432/70‑1 (MQ=255)
              tCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAat                                                            <  1:3965145/64‑1 (MQ=255)
              tCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATctgc                                                     <  1:2409581/71‑1 (MQ=255)
              tCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATctgc                                                     <  1:1478192/71‑1 (MQ=255)
                aCGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAatc                                                           >  1:1731235/1‑63 (MQ=255)
                aCGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATctgctg                                                   >  1:2220525/1‑71 (MQ=255)
                aCGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATctgct                                                    <  1:1933689/70‑1 (MQ=255)
                 cGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATctgct                                                    <  1:500899/69‑1 (MQ=255)
                 cGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTgg                                                  <  1:4128993/71‑1 (MQ=255)
                 cGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTgg                                                  <  1:1413611/71‑1 (MQ=255)
                    tATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAg                                               >  1:1964490/1‑71 (MQ=255)
                    tATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAg                                               >  1:4116366/1‑71 (MQ=255)
                     aTCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGt                                              >  1:3033196/1‑71 (MQ=255)
                      tCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGt                                              <  1:657709/70‑1 (MQ=255)
                      tCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGt                                              <  1:2013234/70‑1 (MQ=255)
                      tCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGt                                              <  1:224928/70‑1 (MQ=255)
                       cAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGt                                              <  1:1433466/69‑1 (MQ=255)
                           gTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAatc                                                           <  1:2263355/52‑1 (MQ=255)
                           gTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATcc                                        >  1:977968/1‑71 (MQ=255)
                           gTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATcc                                        >  1:1132385/1‑71 (MQ=255)
                           gTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATc                                         >  1:2641894/1‑70 (MQ=255)
                            ttttCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAatcat                                                         <  1:2086358/53‑1 (MQ=255)
                            ttttCAGCGGCCTTCACATCACAGCCATTACGCTCTACCGATTGCTGAatcat                                                         <  1:2649559/53‑1 (MQ=255)
                             tttCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTc                                            <  1:2529452/65‑1 (MQ=255)
                             tttCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCt                                      >  1:240593/1‑71 (MQ=255)
                              ttCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCtt                                     >  1:1248236/1‑71 (MQ=255)
                              ttCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCt                                      <  1:894457/70‑1 (MQ=255)
                              ttCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCt                                      <  1:420415/70‑1 (MQ=255)
                              ttCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCt                                      <  1:2018184/70‑1 (MQ=255)
                              ttCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCt                                      <  1:1188175/70‑1 (MQ=255)
                               tCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATctg                                                      <  1:1162397/53‑1 (MQ=255)
                                 aGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCa                                  <  1:551872/71‑1 (MQ=255)
                                 aGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCa                                  <  1:2301020/71‑1 (MQ=255)
                                 aGCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCa                                  <  1:1865620/71‑1 (MQ=255)
                                  gCGGCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCAt                                 >  1:727194/1‑71 (MQ=255)
                                    ggCCTTCACATCACCGCCATTACGCTCTACCGAtt                                                                   >  1:730813/1‑35 (MQ=255)
                                    ggCCTTCACATCACCGCCATTACGCTCTACCGAtt                                                                   >  1:139771/1‑35 (MQ=255)
                                    ggCCTTCACATCACCGCCATTACGCTCTACCGAtt                                                                   >  1:4013014/1‑35 (MQ=255)
                                    ggCCTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATgc                               >  1:1149053/1‑71 (MQ=255)
                                      ccTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATgcg                              >  1:2652788/1‑70 (MQ=255)
                                       cTTCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCAt                                 >  1:2403689/1‑66 (MQ=255)
                                        ttCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCgt                           >  1:1992133/1‑71 (MQ=255)
                                         tCACATCACCGCCATTACGCTCTACCGATTGCTGAatcatc                                                        >  1:3998455/1‑41 (MQ=255)
                                         tCACATCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCgtg                          >  1:3169412/1‑71 (MQ=255)
                                          cacaTCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCgtgt                         >  1:3705338/1‑71 (MQ=255)
                                           acaTCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGt                                              <  1:5034/49‑1 (MQ=255)
                                           acaTCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGt                                              <  1:1454526/49‑1 (MQ=255)
                                           acaTCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGt                                              <  1:3849352/49‑1 (MQ=255)
                                            caTCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCAt                                          >  1:1562351/1‑52 (MQ=255)
                                            caTCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCg                       <  1:2886007/71‑1 (MQ=255)
                                             aTCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCAt                                 <  1:3097007/60‑1 (MQ=255)
                                             aTCACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCAt                                 <  1:3094509/60‑1 (MQ=255)
                                              tCACCGCCATTACGCTCTACCGATTGCTGAatcatc                                                        >  1:628215/1‑36 (MQ=255)
                                               cACCGCGATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCa                                  >  1:3614436/1‑57 (MQ=255)
                                               cACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCa                                           >  1:3622091/1‑48 (MQ=255)
                                               cACCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGt                     >  1:2681339/1‑70 (MQ=255)
                                                aCCGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCa                   <  1:2958651/71‑1 (MQ=255)
                                                 ccGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCAt                  <  1:2684895/71‑1 (MQ=255)
                                                 ccGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCAt                  <  1:632785/71‑1 (MQ=255)
                                                  cGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCAtt                 >  1:3601308/1‑71 (MQ=255)
                                                  cGCCATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCAtt                 >  1:2833644/1‑71 (MQ=255)
                                                     cATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTc                                    <  1:2988422/49‑1 (MQ=255)
                                                     cATTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCTTTTc               <  1:1189727/70‑1 (MQ=255)
                                                      aTTACGCTCTACCGATTGCTGAATCATCTGCTGGa                                                 >  1:2049527/1‑35 (MQ=255)
                                                      aTTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTc               >  1:1269756/1‑69 (MQ=255)
                                                      aTTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGa             <  1:2803519/71‑1 (MQ=255)
                                                      aTTACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGa             <  1:3291751/71‑1 (MQ=255)
                                                       ttACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTc                                    <  1:4053417/47‑1 (MQ=255)
                                                       ttACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTc               <  1:3936174/68‑1 (MQ=255)
                                                       ttACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTc               <  1:3750626/68‑1 (MQ=255)
                                                       ttACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGAc            <  1:2953106/71‑1 (MQ=255)
                                                       ttACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGAc            <  1:2957780/71‑1 (MQ=255)
                                                        tACGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGGCGGTCATTTCGa             <  1:3365631/69‑1 (MQ=255)
                                                         aCGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATccc                                       >  1:2595579/1‑42 (MQ=255)
                                                         aCGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATccc                                       >  1:2091589/1‑42 (MQ=255)
                                                         aCGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACAt          >  1:167094/1‑71 (MQ=255)
                                                         aCGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTAATCCCTTCAATGCGCGTGTCGGTCATTTCGACAt          >  1:3684471/1‑71 (MQ=255)
                                                          cGCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTc               >  1:3353234/1‑65 (MQ=255)
                                                           gCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACAt          <  1:1858043/69‑1 (MQ=255)
                                                           gCTCTACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTAATTTCGACAtgt        <  1:4040077/71‑1 (MQ=255)
                                                               tACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGcc     >  1:1664253/1‑70 (MQ=255)
                                                               tACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGc      >  1:1711280/1‑69 (MQ=255)
                                                               tACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCt    >  1:1241378/1‑71 (MQ=255)
                                                               tACCGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCt    >  1:3490833/1‑71 (MQ=255)
                                                                 ccGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCa                                  >  1:565770/1‑39 (MQ=255)
                                                                 ccGATTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGt  <  1:3864701/71‑1 (MQ=255)
                                                                    |                                                                   
GCGGTCAAGCCGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGT  >  minE/1348734‑1348869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑