| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,461,819 | T→C | pseudogene (136/4605 nt) pseudogene (136/4104 nt) |
yfaS ← yfaS ← |
ECK2220:JW2222+JW2221:b4500; hypothetical protein ECK2220:JW2222:b2228; hypothetical protein, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,461,819 | 0 | T | C | 100.0% | 213.7 / NA | 59 | pseudogene (136/4605 nt) pseudogene (136/4104 nt) | yfaS yfaS | ECK2220:JW2222+JW2221:b4500; hypothetical protein ECK2220:JW2222:b2228; hypothetical protein, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/32); total (27/32) | |||||||||||
CTCTTCACTGCTGCTAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACG > minE/1461751‑1461884 | ctctTCACTGCTGCTAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGc < 1:1416099/71‑1 (MQ=255) cACTGCTGCTAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTgg < 1:800890/69‑1 (MQ=255) cACTGCTGCTAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTgg > 1:201588/1‑69 (MQ=255) cACTGCTGCTAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTgg < 1:3143983/69‑1 (MQ=255) cACTGCTGCTAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTgg > 1:3171485/1‑69 (MQ=255) cACTGCTGCTAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTgg > 1:1679403/1‑69 (MQ=255) cACTGCTGCTAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTgg < 1:3505482/69‑1 (MQ=255) cACTGCTGCTAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTgg > 1:3770932/1‑69 (MQ=255) tgctAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGgaaggaag > 1:1672851/1‑70 (MQ=255) ctAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGc > 1:3407569/1‑58 (MQ=255) ctAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCg < 1:2656241/70‑1 (MQ=255) tAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGaa > 1:910716/1‑71 (MQ=255) aaCTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAatca < 1:2244206/71‑1 (MQ=255) ctgTCAGCAAGCAAAAAGAATGTTCCCCCGGCATGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGc < 1:3493232/71‑1 (MQ=255) ctgTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGc < 1:6657/71‑1 (MQ=255) tgTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCa > 1:2939903/1‑71 (MQ=255) caagcaaAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGc > 1:2734521/1‑70 (MQ=255) aagcaaAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGc > 1:2840252/1‑69 (MQ=255) agcaaAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGaa < 1:933676/54‑1 (MQ=255) gcaaAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGc < 1:2302606/71‑1 (MQ=255) aaaaaGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGcc < 1:831730/70‑1 (MQ=255) aaaaGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTg < 1:1232723/38‑1 (MQ=255) aaaGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAatc < 1:3668829/51‑1 (MQ=255) aaaGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAatc < 1:743723/51‑1 (MQ=255) aaaGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTg < 1:244212/71‑1 (MQ=255) aaaGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTg > 1:2746470/1‑71 (MQ=255) aaGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTg < 1:2964015/70‑1 (MQ=255) aGAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGtt < 1:1645426/71‑1 (MQ=255) gAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTggaa > 1:1781748/1‑37 (MQ=255) gAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCAt < 1:3981477/56‑1 (MQ=255) gAATGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGcc < 1:271026/65‑1 (MQ=255) aaTGTTCCCCCGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAg > 1:1692283/1‑62 (MQ=255) cccccGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCAt < 1:1985554/49‑1 (MQ=255) cccccGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCcac > 1:1598453/1‑68 (MQ=255) cccGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGtt < 1:2650690/61‑1 (MQ=255) cccGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAagag < 1:1546875/71‑1 (MQ=255) cGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAgaga > 1:646706/1‑70 (MQ=255) cGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAgaga > 1:2127261/1‑70 (MQ=255) cGGCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGAc < 1:956443/71‑1 (MQ=255) ggCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTc > 1:181047/1‑59 (MQ=255) ggCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAgaga > 1:3784031/1‑69 (MQ=255) gCAGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAg < 1:2842513/71‑1 (MQ=255) aGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAggg < 1:1596611/71‑1 (MQ=255) aGGCGGCGCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAggg < 1:4054720/71‑1 (MQ=255) gcggcgCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAgaga < 1:3297112/64‑1 (MQ=255) gcggcgCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGAc < 1:601528/71‑1 (MQ=255) cggcgCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCaa > 1:2507524/1‑44 (MQ=255) cggcgCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACa < 1:3158137/71‑1 (MQ=255) cggcgCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACa < 1:1947439/71‑1 (MQ=255) ggcgCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCca > 1:3203856/1‑55 (MQ=255) ggcgCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCca > 1:615331/1‑55 (MQ=255) ggcgCATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGAc > 1:1648636/1‑69 (MQ=255) gcgcATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACaa < 1:960777/70‑1 (MQ=255) gcgcATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAg < 1:1015859/71‑1 (MQ=255) cATAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCAc > 1:1732468/1‑71 (MQ=255) aTAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGcc > 1:95753/1‑41 (MQ=255) aTAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACg > 1:514379/1‑71 (MQ=255) aTAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACg > 1:509540/1‑71 (MQ=255) aTAGTCGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACg > 1:1068008/1‑71 (MQ=255) | CTCTTCACTGCTGCTAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACG > minE/1461751‑1461884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |