Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	128,514	G→A	V25M (GTG→ATG)	yadH →	predicted transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	128,514	0	G	A	100.0%	263.1 / NA	86	V25M (GTG→ATG)	yadH	predicted transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/41);  total (45/41)

TGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATT  >  minE/128446‑128579                                                                     |                                                                  tgCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATg                                                                 <  1:3143382/71‑1 (MQ=255)    aTCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATg                                                                 >  1:1949859/1‑68 (MQ=255)      cTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGAc                                                            >  1:3217376/1‑71 (MQ=255)        ttACTGAGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGc                                                          >  1:538929/1‑71 (MQ=255)         tACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCt                                                         >  1:471628/1‑71 (MQ=255)          aCTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCt                                                         <  1:3277809/70‑1 (MQ=255)           cTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTg                                                        <  1:4142646/70‑1 (MQ=255)           cTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTg                                                        <  1:3761917/70‑1 (MQ=255)              ggTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGt                                                      <  1:182017/69‑1 (MQ=255)              ggTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTg                                                     <  1:847870/70‑1 (MQ=255)                  gcgcTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTgccgcc                                                <  1:1613018/71‑1 (MQ=255)                   cgcTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCa                                               <  1:47075/71‑1 (MQ=255)                    gcTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAg                                              >  1:2468883/1‑71 (MQ=255)                      tAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGc                                                          >  1:986651/1‑57 (MQ=255)                      tAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGt                                             >  1:3832579/1‑70 (MQ=255)                       aaaaaGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGtca                                           >  1:899715/1‑71 (MQ=255)                        aaaaGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGtcat                                          <  1:1549604/71‑1 (MQ=255)                        aaaaGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGtcat                                          <  1:3469613/71‑1 (MQ=255)                        aaaaGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGtcat                                          >  1:3481336/1‑71 (MQ=255)                        aaaaGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGtcat                                          <  1:3538170/71‑1 (MQ=255)                         aaaGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGtcat                                          >  1:649372/1‑70 (MQ=255)                          aaGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGtcatca                                        >  1:2535964/1‑71 (MQ=255)                          aaGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGtcatca                                        >  1:3168389/1‑71 (MQ=255)                          aaGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGtcatca                                        >  1:342008/1‑71 (MQ=255)                           aGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAg                                                              >  1:1069105/1‑48 (MQ=255)                           aGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAg                                                              >  1:1639305/1‑48 (MQ=255)                           aGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAg                                                              >  1:1699533/1‑48 (MQ=255)                            gCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTgccgcc                                                >  1:1131304/1‑61 (MQ=255)                            gCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCAc                                       >  1:279883/1‑70 (MQ=255)                              aTCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCAt                                    <  1:979846/71‑1 (MQ=255)                               tCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTg                                                        <  1:1013730/50‑1 (MQ=255)                               tCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTg                                                     <  1:3426134/53‑1 (MQ=255)                                cTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCAt                                    >  1:3924705/1‑69 (MQ=255)                                cTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGa                                  >  1:3357924/1‑71 (MQ=255)                                cTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGa                                  >  1:1366497/1‑71 (MQ=255)                                cTGGGCGAAAGAGATCCATCGCTTTATCCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGa                                  >  1:3929561/1‑71 (MQ=255)                                 tGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTgcc                                                   >  1:1754911/1‑53 (MQ=255)                                 tGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGa                                  >  1:2865824/1‑70 (MQ=255)                                  tggCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCt                                                         <  1:2447640/45‑1 (MQ=255)                                   ggCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCAt                                    <  1:800018/66‑1 (MQ=255)                                    gCGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCct                              >  1:2713451/1‑71 (MQ=255)                                     cGAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCctt                             <  1:3169131/71‑1 (MQ=255)                                      gAAAGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCcttt                            <  1:1362267/71‑1 (MQ=255)                                       aacgagaTCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCcttt                            <  1:461518/67‑1 (MQ=255)                                        aaGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCctttac                          >  1:1724688/1‑71 (MQ=255)                                        aaGAGATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCctttac                          >  1:2309603/1‑71 (MQ=255)                                        aaGAGATCCATCGATTTATGCGTATCTGGATGCATACGCTGGTGCCGCCAGTCATCACCATGACCctttac                          >  1:2304884/1‑71 (MQ=255)                                         agagATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCctttact                         >  1:54386/1‑71 (MQ=255)                                         agagATCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCctttact                         <  1:3936862/71‑1 (MQ=255)                                          gagaTGCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCctttactt                        <  1:1667389/71‑1 (MQ=255)                                          gagaTCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCctttactt                        >  1:3514904/1‑71 (MQ=255)                                          gagaTCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCctttactt                        >  1:2497235/1‑71 (MQ=255)                                           agaTCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCa                                               >  1:3676499/1‑47 (MQ=255)                                           agaTCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGtca                                           >  1:2574896/1‑51 (MQ=255)                                           agaTCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCctttact                         <  1:465466/69‑1 (MQ=255)                                            gaTCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCctttacttta                      <  1:3361205/71‑1 (MQ=255)                                             aTCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCctttacttta                      <  1:3686161/70‑1 (MQ=255)                                              tCCATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTttat                     >  1:3345098/1‑70 (MQ=255)                                               ccATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTttatt                    >  1:3233191/1‑70 (MQ=255)                                                cATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCctttactt                        <  1:1272517/65‑1 (MQ=255)                                                cATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTttatta                   <  1:3176666/70‑1 (MQ=255)                                                cATCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTttatta                   >  1:2434261/1‑70 (MQ=255)                                                  tCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCctttac                          <  1:2206830/61‑1 (MQ=255)                                                  tCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTttatta                   >  1:3828495/1‑68 (MQ=255)                                                  tCGCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCt                <  1:2879501/71‑1 (MQ=255)                                                  tCGCTTTATGCGTATCTGGATGCAGACGCTGGTGACGCAAGTCATCACCATg                                   >  1:2599647/1‑52 (MQ=255)                                                    gCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCAc                                       >  1:3005494/1‑46 (MQ=255)                                                    gCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCAc                                       >  1:143332/1‑46 (MQ=255)                                                    gCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCtt               <  1:1009059/70‑1 (MQ=255)                                                    gCTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCtt               <  1:2366357/70‑1 (MQ=255)                                                     cTTTATGCGTATCTGGATGCAGACGCTGGTgccgc                                                 <  1:4060403/35‑1 (MQ=255)                                                     cTTTATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCg             <  1:1845423/71‑1 (MQ=255)                                                        tATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGt           <  1:1753784/70‑1 (MQ=255)                                                        tATGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGt           <  1:1550880/70‑1 (MQ=255)                                                         aTGCGTATCTGGATGCAGACGCTGGTGCCGCCAGtcatca                                        >  1:2150726/1‑40 (MQ=255)                                                         aTGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTa          <  1:1277100/70‑1 (MQ=255)                                                          tGCGTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAAc        >  1:325453/1‑71 (MQ=255)                                                             gTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTttatta                   >  1:2514429/1‑57 (MQ=255)                                                             gTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATc                 <  1:1635363/59‑1 (MQ=255)                                                             gTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTg     <  1:712306/71‑1 (MQ=255)                                                             gTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTg     <  1:1743872/71‑1 (MQ=255)                                                             gTATCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTg     <  1:2894385/71‑1 (MQ=255)                                                               aTCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGa    <  1:3525834/70‑1 (MQ=255)                                                                tCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGAtt  >  1:3379848/1‑71 (MQ=255)                                                                tCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGAtt  >  1:2457427/1‑71 (MQ=255)                                                                tCTGGATGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGAt   <  1:3925172/70‑1 (MQ=255)                                                                     |                                                                  TGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATT  >  minE/128446‑128579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑