Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,695,569	C→T	M147I (ATG→ATA)	yfhQ ←	predicted methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,695,569	0	C	T	100.0%	268.7 / NA	87	M147I (ATG→ATA)	yfhQ	predicted methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/47);  total (40/47)

TGCTCGCCGTTTTCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCCATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCA  >  minE/1695504‑1695636                                                                  |                                                                    tGCTCGCCGTTTTCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGc                                                                >  1:825228/1‑71 (MQ=255)  gCTCGCCGTTTTCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGc                                                                >  1:3533812/1‑70 (MQ=255)   cTCGCCGTTTTCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGc                                                                >  1:322418/1‑69 (MQ=255)   cTCGCCGTTTTCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGc                                                                <  1:1269348/69‑1 (MQ=255)   cTCGCCGTTTTCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGc                                                                <  1:1274027/69‑1 (MQ=255)      gCCGTTTTCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAgg                                                            <  1:1656289/70‑1 (MQ=255)        cGTTTTCCTGAGTTGCCAGCCAGGCCATGCGAACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTc                                                         <  1:1759765/71‑1 (MQ=255)         gTTTTCCTGAGTTGCCAGCCAGTCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCa                                                        <  1:818868/71‑1 (MQ=255)         gTTTTCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGc                                                                >  1:834789/1‑63 (MQ=255)         gTTTTCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTct                                                        <  1:1676892/71‑2 (MQ=255)           tttCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGc                                                      >  1:3709273/1‑71 (MQ=255)           tttCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACAGCTATCGCCAGGTTCAGc                                                      >  1:3511955/1‑71 (MQ=255)            ttCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCa                                                        >  1:254561/1‑68 (MQ=255)            ttCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGc                                                      >  1:3687642/1‑70 (MQ=255)            ttCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGc                                                      <  1:4150473/70‑1 (MQ=255)            ttCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGc                                                      >  1:1065673/1‑70 (MQ=255)            ttCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGc                                                      >  1:3136005/1‑70 (MQ=255)             tCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCg                                                     <  1:1491639/70‑1 (MQ=255)              ccTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGa                                                    <  1:1156341/70‑1 (MQ=255)                 gAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTg                                                >  1:1218654/1‑71 (MQ=255)                 gAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTg                                                >  1:2112751/1‑71 (MQ=255)                  aGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGc                                                      >  1:106218/1‑64 (MQ=255)                  aGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTg                                                <  1:898851/70‑1 (MQ=255)                  aGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTg                                                >  1:2659140/1‑70 (MQ=255)                  aGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTg                                                <  1:824450/70‑1 (MQ=255)                  aGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTg                                                <  1:3601481/70‑1 (MQ=255)                  aGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTg                                                <  1:2370486/70‑1 (MQ=255)                  aGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCAGAACCGCTATCGCCAGGTTCAGCGAGCTg                                                >  1:323524/1‑70 (MQ=255)                       ccagccagGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGt                                               <  1:1578163/66‑1 (MQ=255)                       ccagccagGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTcc                                          <  1:1866260/71‑1 (MQ=255)                        cagccagGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCg                                         >  1:408020/1‑71 (MQ=255)                        cagccagGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCg                                         >  1:3509494/1‑71 (MQ=255)                        cagccagGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCg                                         >  1:1303038/1‑71 (MQ=255)                        cagccagGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCg                                         <  1:3591453/71‑1 (MQ=255)                        cagccagGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCg                                         <  1:724382/71‑1 (MQ=255)                        cagccagGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCg                                         <  1:2259780/71‑1 (MQ=255)                        cagccagGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCg                                         <  1:194434/71‑1 (MQ=255)                         agccagGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCgg                                        <  1:400141/71‑1 (MQ=255)                           ccagGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGt                                      <  1:2698769/71‑1 (MQ=255)                           ccagGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGt                                      <  1:2913904/71‑1 (MQ=255)                            cagGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGtt                                     <  1:3825810/71‑1 (MQ=255)                            cagGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGtt                                     <  1:1264188/71‑1 (MQ=255)                             agGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCggg                                       >  1:3613296/1‑68 (MQ=255)                             agGCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGt                                      >  1:3028332/1‑69 (MQ=255)                              ggCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCg                                         >  1:1946094/1‑65 (MQ=255)                              ggCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAg                                   <  1:2265090/71‑1 (MQ=255)                              ggCCATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAg                                   <  1:1609743/71‑1 (MQ=255)                                 cATGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCg                                >  1:2254564/1‑71 (MQ=255)                                  aTGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCgg                                        >  1:1040863/1‑62 (MQ=255)                                   tGCGCACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTc                                           <  1:2894323/58‑1 (MQ=255)                                    gcgcACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCaa                             >  1:462454/1‑71 (MQ=255)                                    gcgcACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCa                              >  1:451372/1‑70 (MQ=255)                                     cgcACTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCaa                             <  1:1387791/70‑1 (MQ=255)                                        aCTTCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATcg                          >  1:3361891/1‑70 (MQ=255)                                          ttCATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGa                       >  1:3105342/1‑71 (MQ=255)                                            cATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATcgcg                        <  1:919886/68‑1 (MQ=255)                                            cATAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGAc                      <  1:3122695/70‑1 (MQ=255)                                             aTAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATcgcg                        <  1:2498947/67‑1 (MQ=255)                                             aTAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACa                     <  1:3685037/70‑1 (MQ=255)                                             aTAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACAt                    >  1:2132352/1‑71 (MQ=255)                                              tAGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATg                   <  1:3583526/71‑1 (MQ=255)                                               aGGCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTg                                                <  1:1266626/41‑1 (MQ=255)                                                ggCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGAt                 <  1:2537093/71‑1 (MQ=255)                                                 gCAATAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATa                <  1:2610110/71‑1 (MQ=255)                                                   aaTAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAAt              >  1:2866840/1‑71 (MQ=255)                                                    aTAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGAt                 <  1:1780771/67‑1 (MQ=255)                                                    aTAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATg             <  1:3959628/71‑1 (MQ=255)                                                     tAACCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATgg            >  1:1368789/1‑71 (MQ=255)                                                      aaCCTGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATgg            >  1:1419869/1‑70 (MQ=255)                                                        ccTGAACCGCTATCGCCAGGTTCAGCGAGCTGTAtt                                            <  1:3452275/36‑1 (MQ=255)                                                         cTGAACCGCTATCGCCAGGTTCAGCGAGCTGTAtt                                            <  1:2661830/35‑1 (MQ=255)                                                          tGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCAttt       >  1:2489539/1‑71 (MQ=255)                                                          tGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCAttt       >  1:3294105/1‑71 (MQ=255)                                                          tGAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCAttt       >  1:1630757/1‑71 (MQ=255)                                                           gAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTc      >  1:571965/1‑71 (MQ=255)                                                           gAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTc      >  1:2976983/1‑71 (MQ=255)                                                           gAACCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTc      >  1:3700675/1‑71 (MQ=255)                                                            aaCCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACa                     >  1:4070679/1‑55 (MQ=255)                                                            aaCCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACa                     >  1:1687346/1‑55 (MQ=255)                                                            aaCCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCAttt       <  1:409728/69‑1 (MQ=255)                                                            aaCCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCAttt       <  1:1316896/69‑1 (MQ=255)                                                            aaCCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCt     <  1:1349705/71‑1 (MQ=255)                                                            aaCCGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCt     >  1:2969782/1‑71 (MQ=255)                                                              ccGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGc   <  1:1654055/71‑1 (MQ=255)                                                              ccGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGc   <  1:690277/71‑1 (MQ=255)                                                              ccGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGc   <  1:694929/71‑1 (MQ=255)                                                               cGCTATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATCTCTGCa  <  1:3737996/71‑1 (MQ=255)                                                                  |                                                                    TGCTCGCCGTTTTCCTGAGTTGCCAGCCAGGCCATGCGCACTTCATAGGCAATAACCTGAACCGCCATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCA  >  minE/1695504‑1695636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑