Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,869,315	T→C	K147K (AAA→AAG)	ygcW ←	predicted deoxygluconate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,869,315	0	T	C	100.0%	353.9 / NA	96	K147K (AAA→AAG)	ygcW	predicted deoxygluconate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/58);  total (38/58)

CAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGG  >  minE/1869254‑1869381                                                              |                                                                   cAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCtt                                                           <  1:1504522/71‑1 (MQ=255)    aaTATGCAGGTGACCATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTc                                                        >  1:2430976/1‑71 (MQ=255)        tGCAGGTGACCATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGc                                                     <  1:102376/70‑1 (MQ=255)        tGCAGGTGACCATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGc                                                     <  1:994203/70‑1 (MQ=255)        tGCAGGTGACCATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGc                                                     <  1:992386/70‑1 (MQ=255)        tGCAGGTGACCATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCg                                                    >  1:83862/1‑71 (MQ=255)        tGCAGGTGACCATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCg                                                    <  1:4008725/71‑1 (MQ=255)          cAGGTGACCATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCgg                                                   <  1:1273433/70‑1 (MQ=255)          cAGGTGACCATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCgg                                                   <  1:3799087/70‑1 (MQ=255)             gTGACCATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGAt                                                >  1:2127742/1‑70 (MQ=255)              tGACCATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCa                                              >  1:2537116/1‑71 (MQ=255)               gACCATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCg                                                              >  1:4073171/1‑54 (MQ=255)                aCCATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATa                                            <  1:2354085/71‑1 (MQ=255)                 ccATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATaa                                           <  1:3089023/71‑1 (MQ=255)                  cATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGcc                                                               <  1:2752807/50‑1 (MQ=255)                  cATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGcc                                                               <  1:1894394/50‑1 (MQ=255)                  cATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCgg                                                   <  1:984596/62‑1 (MQ=255)                  aaTTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGcc                                                               <  1:3053089/49‑1 (MQ=255)                    ttGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCtttt                                                         >  1:2953294/1‑54 (MQ=255)                    ttGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTg                                                      >  1:199947/1‑57 (MQ=255)                    ttGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAAttt                                        <  1:3871273/71‑1 (MQ=255)                    ttGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAAttt                                        <  1:3752596/71‑1 (MQ=255)                    ttGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAAttt                                        <  1:1054017/71‑1 (MQ=255)                    ttGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAAttt                                        <  1:3747750/71‑1 (MQ=255)                    ttGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAAtt                                         >  1:3029014/1‑70 (MQ=255)                    ttGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAAtt                                         >  1:61773/1‑70 (MQ=255)                     tGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAAtttt                                       >  1:223189/1‑71 (MQ=255)                     tGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAAtttt                                       >  1:355390/1‑71 (MQ=255)                         cACCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCa                                   <  1:4008613/71‑1 (MQ=255)                          aCCTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCt                                                       <  1:1815728/50‑1 (MQ=255)                            cTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCt                                <  1:3292142/71‑1 (MQ=255)                            cTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCt                                <  1:503756/71‑1 (MQ=255)                            cTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCt                                >  1:1981436/1‑71 (MQ=255)                            cTAAGTAAGAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCt                                <  1:2810160/71‑1 (MQ=255)                             taagtaagAGTACAATTAACAGATATTAATAATCTTTCCGCTTTTCTGCGGGATCATAAttt                                        <  1:2068250/62‑1 (MQ=255)                             taagtaagAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATaa                                           >  1:2208817/1‑59 (MQ=255)                             taagtaagAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAAt                                          >  1:2474326/1‑60 (MQ=255)                             taagtaagAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCtt                               >  1:1185035/1‑71 (MQ=255)                             taagtaagAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCtt                               >  1:1339038/1‑71 (MQ=255)                              aagtaagAGAACAATGAACAGATATTAATAATCTTGCCGCtt                                                           >  1:995426/1‑42 (MQ=255)                               agtaagAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCtt                               <  1:3902481/69‑1 (MQ=255)                               agtaagAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCtt                               <  1:3080663/69‑1 (MQ=255)                               agtaagAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCtt                               <  1:3351017/69‑1 (MQ=255)                                gtaagAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCa                             <  1:2554168/70‑1 (MQ=255)                                gtaagAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCAt                            <  1:1274389/71‑1 (MQ=255)                                gtaagAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCAt                            <  1:398153/71‑1 (MQ=255)                                 taagAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATa                           <  1:3256828/71‑1 (MQ=255)                                  aagAGAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAg                          >  1:830661/1‑71 (MQ=255)                                   agagAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCa                             >  1:146332/1‑67 (MQ=255)                                   agagAACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGc                         >  1:740519/1‑71 (MQ=255)                                    gagaACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAAtttt                                       >  1:2475193/1‑56 (MQ=255)                                    gagaACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCtt                               >  1:2475965/1‑64 (MQ=255)                                    gagaACAATGAACAGATATAAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCtt                               >  1:176922/1‑64 (MQ=255)                                     agaACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCtt                       <  1:177906/71‑1 (MQ=255)                                      gaACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAAtttt                                       <  1:3571681/54‑1 (MQ=255)                                      gaACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCt                                <  1:3339765/61‑1 (MQ=255)                                      gaACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCt                                <  1:3138856/61‑1 (MQ=255)                                      gaACAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTa                      <  1:619605/71‑1 (MQ=255)                                       aaCAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAAttt                                        <  1:3345343/52‑1 (MQ=255)                                       aaCAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAAttt                                        <  1:2675852/52‑1 (MQ=255)                                       aaCAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTaa                     <  1:2286410/71‑1 (MQ=255)                                         cAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCg                                                    >  1:791051/1‑38 (MQ=255)                                         cAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCg                                                    >  1:2695784/1‑38 (MQ=255)                                         cAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAAc                    >  1:3048728/1‑70 (MQ=255)                                         cAATGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACt                   <  1:718241/71‑1 (MQ=255)                                          aaTGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTc                  <  1:411372/71‑1 (MQ=255)                                          aaTGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTc                  <  1:406514/71‑1 (MQ=255)                                          aaTGAACAGATATTAATAATCTTGACGCTTTTCTGCGGGATCATaa                                           <  1:1744508/46‑1 (MQ=255)                                           aTGAACAGATATTAATAATCTTGCCGCTTTTCTGCgg                                                   <  1:2380745/37‑1 (MQ=255)                                            tGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGAt                                                >  1:448079/1‑39 (MQ=255)                                            tGAACAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGa                <  1:2973153/71‑1 (MQ=255)                                               aCAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATg             >  1:2270121/1‑71 (MQ=255)                                               aCAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATg             >  1:1513499/1‑71 (MQ=255)                                                cAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATgc            <  1:3358319/71‑1 (MQ=255)                                                cAGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATgc            >  1:2513691/1‑71 (MQ=255)                                                 aGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTaa                     >  1:740556/1‑61 (MQ=255)                                                 aGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTaa                     >  1:2890405/1‑61 (MQ=255)                                                 aGATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTaa                     >  1:3610165/1‑61 (MQ=255)                                                  gATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGCATgcgg          <  1:539676/71‑1 (MQ=255)                                                  gATATTAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATgcgg          <  1:1693750/71‑1 (MQ=255)                                                   atatTAATAATCTTGCCGCTTTTCTGCGGGATCATaa                                           >  1:81626/1‑37 (MQ=255)                                                     atTAATAATCTTGCCGCTTTTCTGCGGGATCATAAttttt                                      >  1:793155/1‑40 (MQ=255)                                                      ttAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGcggcgg       <  1:1011600/70‑1 (MQ=255)                                                      ttAATAATCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGt      <  1:526560/71‑1 (MQ=255)                                                       taataaTCTTGCCGCTTTTCTGCGGGATCATAATTTTTGc                                    <  1:3376219/40‑1 (MQ=255)                                                        aataatCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCg                 <  1:3076050/58‑1 (MQ=255)                                                        aataatCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGt      >  1:3273848/1‑69 (MQ=255)                                                         ataatCTTGCCGCTTTTCTGCGGGATCATAATTTTTGc                                    >  1:694289/1‑38 (MQ=255)                                                         ataatCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAg   <  1:4015464/71‑1 (MQ=255)                                                         ataatCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAg   <  1:4020211/71‑1 (MQ=255)                                                          taatCTTGCCGCTTTTCTGCTGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAgg  <  1:104880/71‑1 (MQ=255)                                                          taatCTTGCCGCTTTTCTGCGGGATCATAAttttt                                      >  1:3771089/1‑35 (MQ=255)                                                          taatCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATgcgg          <  1:1031951/63‑1 (MQ=255)                                                          taatCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAgg  <  1:3751230/71‑1 (MQ=255)                                                          taatCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAgg  <  1:270762/71‑1 (MQ=255)                                                          taatCTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAgg  <  1:104876/71‑1 (MQ=255)                                                              |                                                                   CAGAATATGCAGGTGACCATTGTCCACCTAAGTAAGAGAACAATGAACAGATATTAATAATTTTGCCGCTTTTCTGCGGGATCATAATTTTTGCAGCTTCATAGCTTAACTCGAATGCGGCGGTCAGG  >  minE/1869254‑1869381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑