Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,143,361	T→C	noncoding (22/77 nt)	metY ←	tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,143,361	0	T	C	100.0%	160.4 / NA	44	noncoding (22/77 nt)	metY	tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/18);  total (26/18)

AGGGAAAGTGGTTGCGGGGGCCGGATTTGAACCGACGATCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAA  >  minE/2143298‑2143427                                                                |                                                                   aGGGAAAGTGGTTGCGGGGGCCGGATTTGAACCGACGATCTTCGGGTTATGAGCCCGACGAGCCACCAGgc                                                             >  1:2812598/1‑71 (MQ=255) aGGGAAAGTGGTTGCGGGGGCCGGATTTGAACCGACGATCTTCGGGTTATGAGCCCGACGAGCCACCAGgc                                                             >  1:1317951/1‑71 (MQ=255)    gAAAGTGGTTGCGGGGGCCGGATTTGAACCGACGATCTTCGGGTTATGAGCCCGACGAGCCACCAGgctgc                                                          <  1:1942762/71‑1 (MQ=255)      aaGTGGTTGCGGGGGCCGGATTTGAACCGACGATCTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTc                                                        >  1:2456385/1‑71 (MQ=255)           gTTGCGGGGGCCGGATTTGAACCGACGATCTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCAcc                                                    <  1:1992305/70‑1 (MQ=255)              gCGGGGGCCGGATTTGAACCGACGATCTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCcgc                                                >  1:2387462/1‑71 (MQ=255)                     ccGGATTTGAACCGACGATCTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTc                                                        <  1:2665847/56‑1 (MQ=255)                       ggATTTGAACCGACGATCTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCt                                            >  1:2169032/1‑66 (MQ=255)                        gATTTGAACCGACGATCTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACg                                      >  1:1700715/1‑71 (MQ=255)                        gATTTGAACCGACGATATTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAAc                                       <  1:3140692/70‑1 (MQ=17)                         aTTTGAACCGACGATCTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCAcccc                                                  <  1:155687/58‑1 (MQ=255)                         aTTTGAACCGACGATCTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGt                                     <  1:1541460/71‑1 (MQ=255)                         aTTTGAACCGACGATCTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGt                                     <  1:1997947/71‑1 (MQ=255)                           ttGAACCGACGATCTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACg                                      <  1:324164/68‑1 (MQ=255)                              aaCCGACGATCTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCa                                 <  1:304837/70‑1 (MQ=255)                               aCCGACGATCTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCaaa                               >  1:2579335/1‑71 (MQ=255)                                ccGACGATCTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTgg                                   >  1:1330318/1‑66 (MQ=255)                                    cgaTCTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGCCAAATTCTa                          <  1:624938/71‑1 (MQ=21)                                     gaTCTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCcgc                                                <  1:1423687/48‑1 (MQ=255)                                     gaTCTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTAc                         >  1:311926/1‑71 (MQ=255)                                      aTCTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTAc                         >  1:121865/1‑70 (MQ=255)                                      aTCTTCGGGTTATGAGCCCGACGAGACACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACt                        >  1:966995/1‑71 (MQ=255)                                        cTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCg                      >  1:1690751/1‑71 (MQ=255)                                        cTTCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCg                      >  1:3485312/1‑71 (MQ=255)                                         ttCGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTc                            <  1:64608/64‑1 (MQ=255)                                           cGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTc                       <  1:2612577/67‑1 (MQ=255)                                           cGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGttt                   >  1:3852270/1‑71 (MQ=255)                                           cGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGtt                    <  1:2370980/70‑1 (MQ=255)                                           cGGGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGt                     <  1:2195790/69‑1 (MQ=255)                                               ttATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTg                                    >  1:1018409/1‑50 (MQ=255)                                                 aTGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGtttt                  >  1:1133797/1‑66 (MQ=255)                                                  tGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTa             >  1:822397/1‑70 (MQ=255)                                                  tGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTa             >  1:1021287/1‑70 (MQ=255)                                                    aGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTaaaa          <  1:673755/71‑1 (MQ=255)                                                    aGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTaaa           >  1:3065827/1‑70 (MQ=255)                                                    aGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTaaa           >  1:1487139/1‑70 (MQ=255)                                                    aGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTaaa           >  1:1278272/1‑70 (MQ=255)                                                      cccGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTaaaaaa        >  1:594474/1‑71 (MQ=255)                                                      cccGACCAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTaaaaaa        >  1:185/1‑71 (MQ=255)                                                       ccGACGAGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAAt       >  1:3554282/1‑71 (MQ=255)                                                         gacgaGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATg      >  1:2122396/1‑70 (MQ=255)                                                           cgaGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTTGCAAATTCTACTCGTTTTGGGTAAAAAATGCa    >  1:2221684/1‑70 (MQ=255)                                                            gaGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCaaa  <  1:1668196/71‑1 (MQ=255)                                                            gaGCCACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCaaa  <  1:989771/71‑1 (MQ=255)                                                                |                                                                   AGGGAAAGTGGTTGCGGGGGCCGGATTTGAACCGACGATCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAA  >  minE/2143298‑2143427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑